{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:14Z","timestamp":1786977554779,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010801","name":"Xunta de Galicia","doi-asserted-by":"publisher","award":["2021\u2010PG054"],"award-info":[{"award-number":["2021\u2010PG054"]}],"id":[{"id":"10.13039\/501100010801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Gamma\u2010hydroxybutyrate (GHB), an endogenous compound related to the neurotransmitter gamma\u2010aminobutyric acid (GABA), is used as a therapeutic and recreational drug and as a \u201cweapon\u201d in drug\u2010facilitated crimes. The very short window of detection of GHB in conventional matrices (blood and urine) makes necessary the use of alternative matrices like hair. Hair has a long window of detection and the possibility to perform segmental analysis, which makes it very useful for proving GHB intake. In the present work, a method for quantification of GHB in hair was developed and validated. Hair (10\u2009mg) was washed twice with dichloromethane and then incubated at room temperature with Milli\u2010Q water in an ultrasound bath for 30\u2009min. Analysis was performed by UPLC\u2010MS\/MS using a CORTECS UPLC HILIC (1.6\u2009\u03bcm), 2.1\u2009\u00d7\u2009100\u2010mm column, and a gradient with acetonitrile and ammonium acetate (10\u2009mM) at pH\u20096.0, with a total run\u2010time of 10\u2009min. For detection, a triple quadrupole mass spectrometer in ESI negative mode was used. The method was validated, following the criteria established in the \u201cAAFS Standard Practices for Method Validation in Forensic Toxicology\u201d guideline, obtaining satisfactory results for linearity (0.5\u201350\u2009ng\/mg), accuracy (95.0%\u2013103.2%), imprecision (&lt;\u200910.2%), limit of detection (0.1\u2009ng\/mg) and quantification (0.5\u2009ng\/mg), exogenous selectivity (no interferences), matrix effect (less than \u221244.2%), extraction efficiency (&gt;\u200986.4%), process efficiency (&gt;\u200946.1%), and autosampler stability (&lt;\u20094.3%). The method was used for the analysis of 26 authentic hair samples, 25 from non\u2013drug users, obtaining values between &lt;\u2009LOQ and 6.25\u2009ng\/mg of endogenous GHB and 1 from a former GHB chronic user to prove abstinence.<\/jats:p>","DOI":"10.1002\/dta.3798","type":"journal-article","created":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T23:49:47Z","timestamp":1726444187000},"page":"903-914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid Analytical Method for Quantification of Gamma\u2010Hydroxybutyrate (GHB) in Hair by UPLC\u2010MS\/MS"],"prefix":"10.1002","volume":"17","author":[{"given":"Miriam","family":"Blanco\u2010Ces","sequence":"first","affiliation":[{"name":"Toxicology Service Institute of Forensic Sciences, University of Santiago de Compostela  Santiago de Compostela 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