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In spite of its yet non\u2010authorized use as a medicine and being forbidden by the IFHA, the FEI, and the WADA, intelligence and doping control laboratories reported numerous products available online claiming to contain a synthetic acetylated fragment of TB4 or TB4 itself, promoted as a growth factor with regenerative properties. In this article, the first estimation of the endogenous TB4 concentration in racing horses\u2019 blood samples was performed through a population study. We reveal that this concentration does not significantly depend on gender, age, nor horse breed. We highlight that the TB4 concentration increases significantly and rapidly in plasma stored at 4\u00b0C when not separated from blood cells due to cell lysis. Finally, we also demonstrate that the detection of a non\u2010natural synthesis impurity is possible in equine plasma after a single dose administration of a TB4 containing product to a horse.<\/jats:p>","DOI":"10.1002\/dta.3806","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T03:23:06Z","timestamp":1727148186000},"page":"1071-1077","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Equine Doping Controls of Thymosin \u03b24: A Population Study and Strategy for Misuse Detection"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3840-0400","authenticated-orcid":false,"given":"Vivian","family":"Delcourt","sequence":"first","affiliation":[{"name":"GIE LCH, Laboratoire des Courses Hippiques  Verri\u00e8res\u2010le\u2010Buisson 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