{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T10:20:55Z","timestamp":1776680455517,"version":"3.51.2"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T00:00:00Z","timestamp":1734134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study presents the development and validation of a drug testing method in hair, employing a semi\u2010automated sample preparation procedure and gas chromatography\u2013tandem mass spectrometry (GC\u2013MS\/MS) for simultaneous multidrug analysis in hair. The method aims to detect and quantify multiple drugs in hair, including amphetamine, methamphetamine, 3,4\u2010methylenedioxyamphetamine, 3,4\u2010methylenedioxymethamphetamine, ketamine, norketamine, cocaine, benzoylecgonine, morphine, codeine and 6\u2010monoacetylmorphine, using a comprehensive procedure involving decontamination, pulverization, clean\u2010up using supported\u2010liquid extraction (SLE) and a two\u2010step derivatization process. The method validation included specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, carryover, robustness and stability. The results indicate a linearity range of 0.1\u20135\u2009ng\/mg for all drugs except methamphetamine, which has a linearity range of 0.2\u20135\u2009ng\/mg. The accuracy and precision of the method are within \u00b120%, with the LOQ at 0.2\u2009ng\/mg for methamphetamine, 0.1\u2009ng\/mg for amphetamine and MDMA and 0.05\u2009ng\/mg for the rest of the drugs. The LODs were found to be 0.1\u2009ng\/mg for methamphetamine, 0.05\u2009ng\/mg for amphetamine and MDMA and 0.025\u2009ng\/mg for other drugs. This method has demonstrated its accuracy by comparing the quantitative test results with the expected results, using hair samples from the proficiency test service providers. This test method offers efficient testing of multiple drugs in a single analysis, significantly improves the hair analysis workflow in a routine testing laboratory by reducing turnaround time and the amount of hair sample required.<\/jats:p>","DOI":"10.1002\/dta.3840","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T03:33:12Z","timestamp":1734147192000},"page":"1344-1356","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An Improved GC\u2013MS\/MS Method for a Fast Multidrug Analysis in Hair"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1686-2708","authenticated-orcid":false,"given":"HuiFen\u00a0Hannah","family":"Zhang","sequence":"first","affiliation":[{"name":"Analytical Toxicology Laboratory, Analytical Toxicology Division Health Sciences Authority  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u00a0Ying\u00a0Hilda","family":"Loh","sequence":"additional","affiliation":[{"name":"Analytical Toxicology Laboratory, Analytical Toxicology Division Health Sciences Authority  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hooi\u00a0Yan","family":"Moy","sequence":"additional","affiliation":[{"name":"Analytical Toxicology Laboratory, Analytical Toxicology Division Health Sciences Authority  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u00a0Pang","family":"Lui","sequence":"additional","affiliation":[{"name":"Analytical Toxicology Laboratory, Analytical Toxicology Division Health Sciences Authority  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,14]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Hair Analysis in Clinical and Forensic Toxicology","author":"Kintz P.","year":"2015"},{"key":"e_1_2_8_3_1","unstructured":"\u201cMisuse of Drugs Act. Misuse of Drugs (Hair Specimens and Hair Tests) Regulations \u201d Cap. 185 Singapore Statutes Online 2013 accessed September 6 2024 https:\/\/sso.agc.gov.sg\/SL\/MDA1973\u2010S269\u20102013?DocDate=20130430."},{"key":"e_1_2_8_4_1","unstructured":"United Nations Office on Drugs and Crime \u201cSynthetic Drugs in East and Southeast Asia: Latest Developments and Challenges \u201d UNODC Regional Office for Southeast Asia and the Pacific 2024 accessed September 6 2024 https:\/\/www.unodc.org\/roseap\/uploads\/documents\/Publications\/2024\/Synthetic_Drugs_in_East_and_Southeast_Asia_2024.pdf."},{"key":"e_1_2_8_5_1","unstructured":"Central Narcotics Bureau \u201cDrug Situation Report \u201d Singapore 2023 accessed September 6 2024 https:\/\/www.cnb.gov.sg\/docs\/default\u2010source\/drug\u2010situation\u2010report\u2010documents\/cnb\u2010annual\u2010statistics\u20102023\u2010(final).pdf?sfvrsn=95b37b39_4."},{"key":"e_1_2_8_6_1","unstructured":"United Nations Office on Drugs and Crime \u201cWorld Drug Report 2024: Drug Market Patterns and Trends \u201d Vienna Austria: United Nations Publication 2024 accessed September 6 2024 https:\/\/www.unodc.org\/unodc\/en\/data\u2010and\u2010analysis\/wdr2024\u2010drug\u2010market\u2010trends.html."},{"key":"e_1_2_8_7_1","unstructured":"United Nations Office on Drugs and Crime \u201cGlobal Report on Cocaine 2023 \u201d Vienna Austria: United Nations Publication 2023 accessed September 6 2024 https:\/\/www.unodc.org\/documents\/data\u2010and\u2010analysis\/cocaine\/Global_cocaine_report_2023.pdf."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2017.08.031"},{"key":"e_1_2_8_9_1","unstructured":"\u201cBiotage.ISOLUTE\u00ae SLE+ User GuideSupported Liquid Extraction\u201d (Sweden: Biotage 2018) accessed February 13 2024 https:\/\/www.biotage.com\/hubfs\/Archive\/UngatedPDF\/ui304.v4_sle_user_guide.pdf."},{"key":"e_1_2_8_10_1","unstructured":"\u201cBiotage.Streamlined Sample Preparation of a Drugs of Abuse Panel in Human Hair Using ISOLUTE\u00ae SLE+ Prior to UPLC\u2010MS\/MS Analysis \u201d Application Note AN897. 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