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Its pharmacological properties are currently undefined, and it is yet to be found in biological samples. However, it is unclear whether this is due to low prevalence or hampered detection. The aim of this study was twofold. First, we used a powder seized by customs as a case study to evaluate the utility of low\u2010field nuclear magnetic resonance (LF\u2010NMR) to unequivocally identify CH\u2010FUBBMPDORA. This demonstrated the potential of this technique, which is increasingly used by customs and forensic laboratories for substance identification. High\u2010field nuclear magnetic resonance (HF\u2010NMR), Fourier transform infrared spectrometry (FTIR), gas chromatography\u2013mass spectrometry (GC\u2013MS), liquid chromatography coupled to time\u2010of\u2010flight mass spectrometry (LC\u2010QTOF\u2010MS), and Raman spectroscopy were used as complementary techniques for identification and characterization. Second, we investigated the potential to activate CB<jats:sub>1<\/jats:sub> and CB<jats:sub>2<\/jats:sub> and the metabolism of CH\u2010FUBBMPDORA. Potencies and efficacies were assessed using \u03b2arr2 recruitment assays. Metabolite studies were conducted via human liver microsome (HLM) incubation followed by LC\u2010QTOF\u2010MS. CH\u2010FUBBMPDORA showed a limited activation potential at both cannabinoid receptors. The seized powder exhibited a pronouncedly higher activity, suggesting the potential presence of contaminants with higher cannabinoid activity. Although analytical characterization revealed minor impurities, it is uncertain whether these explain the bioassay findings. Four metabolites were identified, which were all the result of hydroxylation of either the cyclohexyl head group or of the methyl group on the core.<\/jats:p>","DOI":"10.1002\/dta.3854","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T02:39:06Z","timestamp":1739414346000},"page":"1623-1639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification and Investigation of the Intrinsic Receptor Activation Potential and Metabolization of the New Oxo\u2010Pyridyl Synthetic Cannabinoid Receptor Agonist CH\u2010FUBBMPDORA"],"prefix":"10.1002","volume":"17","author":[{"given":"Marie\u00a0H.","family":"Deventer","sequence":"first","affiliation":[{"name":"Laboratory of Toxicology, Department of Bioanalysis, Faculty of Pharmaceutical Sciences Ghent University  Ghent 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