{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:47:40Z","timestamp":1771462060039,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Dietary supplements used by recreational and elite athletes for performance enhancement might contain undeclared, unlawfully added ingredients. One of those ingredients is 5\u03b1\u2010hydroxy\u2010laxogenin, which is sold in dietary supplements marketed as a natural compound with anabolic effects. It has been shown that 5\u03b1\u2010hydroxy\u2010laxogenin is not naturally occurring, but rather of synthetic origin. Previously, we observed that 5\u03b1\u2010hydroxy\u2010laxogenin can bind to and activate the androgen receptor in a cell\u2010based bioassay. To investigate its androgenic potential in\u00a0vivo, we treated orchiectomized rats with three different dosages of 5\u03b1\u2010hydroxy\u2010laxogenin for 2 weeks. Effects were neither observed on the wet weights of the androgen target tissues prostate, seminal vesicle or penis nor on the wet weights of the anabolic target tissue <jats:italic>musculus levator ani<\/jats:italic> or on skeletal hindlimb muscles. Au contraire, significantly higher atrophy was seen for some of the target tissues in the animals treated with the highest 5\u03b1\u2010hydroxy\u2010laxogenin dosage (36\u2009mg\/kg bw). While in silico docking supports the androgen receptor binding previously observed in\u00a0vitro, we observed neither androgenic nor anabolic effects of 5\u03b1\u2010hydroxy\u2010laxogenin in\u00a0vivo in castrated male rats.<\/jats:p>","DOI":"10.1002\/dta.3881","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:28:47Z","timestamp":1741753727000},"page":"1743-1749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of the Dietary Supplement 5\u03b1\u2010Hydroxy\u2010Laxogenin in the Orchiectomized Rat Model"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4162-1028","authenticated-orcid":false,"given":"Daniel","family":"Derwand","sequence":"first","affiliation":[{"name":"Institute of Doping Analysis and Sports Biochemistry Dresden  Kreischa Germany"},{"name":"Faculty of Biology, Environmental Monitoring &amp; Endocrinology Technische Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4480-1446","authenticated-orcid":false,"given":"Oliver","family":"Zierau","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Environmental Monitoring &amp; Endocrinology Technische Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemens\u00a0Alexander","family":"Wolf","sequence":"additional","affiliation":[{"name":"Molecular Design Lab, Institute of Pharmacy, Department of Biology, Chemistry and Pharmacy Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5344-0048","authenticated-orcid":false,"given":"Gerhard","family":"Wolber","sequence":"additional","affiliation":[{"name":"Molecular Design Lab, Institute of Pharmacy, Department of Biology, Chemistry and Pharmacy Freie Universit\u00e4t Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2157-4711","authenticated-orcid":false,"given":"Annekathrin\u00a0Martina","family":"Keiler","sequence":"additional","affiliation":[{"name":"Institute of Doping Analysis and Sports Biochemistry Dresden  Kreischa Germany"},{"name":"Faculty of Biology, Environmental Monitoring &amp; Endocrinology Technische Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8387271"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3517"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1123\/ijsnem.2023-0263"},{"key":"e_1_2_8_5_1","unstructured":"Agency WA\u2010D \u201cThe Prohibited List \u2010 World Anti\u2010Doping Code International Standard \u201d inAgency WA\u2010D ed (2024)."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9081012"},{"key":"e_1_2_8_7_1","unstructured":"\u201dInformation on Select Dietary Supplement Ingredients and Other Substances \u201d (2024) https:\/\/www.fda.gov\/food\/dietary\u2010supplements\/information\u2010select\u2010dietary\u2010supplement\u2010ingredients\u2010and\u2010other\u2010substances."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.12.045"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.2818"},{"key":"e_1_2_8_10_1","first-page":"05","article-title":"Illegal Ingredient, 5\u2010Alpha\u2010Hydroxy\u2010Laxogenin, Appearing in More Supplements [Press Release]","volume":"25","year":"2022","journal-title":"USADA"},{"key":"e_1_2_8_11_1","unstructured":"Agency WA\u2010D \u201cWorld Anti\u2010Doping Code \u201d inWADA ed (2021)."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-022-03283-5"},{"key":"e_1_2_8_13_1","unstructured":"OECD \u201cOECD Guideline for the Testing of Chemicals \u2010 Hershberger Bioassay in Rats: A Short\u2010Term Screening Assay for (Anti)Androgenic Properties \u201d inOECD ed (2009)."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci100031x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm0603365"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705524200"},{"key":"e_1_2_8_18_1","volume-title":"Chemical Computing Group ULC: 1010 Sherbooke St. West","author":"Molecular Operating Environment (MOE)","year":"2020"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22234"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.051905906"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0897"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(199604)17:5\/6<587::AID-JCC4>3.0.CO;2-Q"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci049885e"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.085779"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00130.2007"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2022.113299"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-012422-112116"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-022-01794-7"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1999.87.6.2016"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1677\/JME-09-0048"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.122861"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfj009"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(00)00125-4"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(00)00094-7"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3881","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T07:37:59Z","timestamp":1761032279000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.3881"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,4]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/dta.3881"],"URL":"https:\/\/doi.org\/10.1002\/dta.3881","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,4]]},"assertion":[{"value":"2024-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}