{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T01:55:48Z","timestamp":1765331748112,"version":"3.46.0"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Clenbuterol (Clb) is a \u03b22\u2010agonist drug included in the list of substances prohibited during and out of competition by the World Anti\u2010Doping Agency (WADA\u2010AMA). Several adverse analytical findings have been detected by accredited WADA laboratories, but athletes often claim that results are due to dietary contamination. In this contribution, bovine microsomal incubation and the excretion of bovine and human urinary metabolites of Clb were analyzed and compared using liquid chromatography electrospray Q\u2010Exactive\u2010Orbitrap mass spectrometry to determine differences in Clb metabolism. Urine samples were processed by solid\u2010phase extraction prior to electrospray analysis in both the positive and negative ion modes. MS\/MS experiments were obtained by parallel monitoring reaction (PRM) triggered by an inclusion ions list. The strategy for metabolite identification involved the search for typical biotransformation based on accurate mass shifts using diagnostic fragment ions from the parent drug. This approach successfully identified eight metabolites, including a novel\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylated form of Clb, reported here for the first time. Additionally, four metabolites found exclusively in bovine urine offer significant potential for further research aimed at distinguishing unintentional doping.\n                  <\/jats:p>","DOI":"10.1002\/dta.3942","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T04:59:11Z","timestamp":1756270751000},"page":"2314-2322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biosynthesis and Identification of Clenbuterol Metabolites in Urine and In\u00a0Vitro Microsome Incubation Samples Using UHPLC\u2010Q\u2010Exactive Orbitrap Mass Spectrometry: A Comparison Between Human and Bovine Metabolism"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9953-9433","authenticated-orcid":false,"given":"Anuar","family":"G\u00f3mez\u2010Tagle","sequence":"first","affiliation":[{"name":"Organic Chemistry Section, Chemical Sciences Department, Laboratory of Medicinal Green Chemistry, Faculty of Higher Studies Cuautitlan National Autonomous University of Mexico  Cuautitl\u00e1n Izcalli Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4153-6656","authenticated-orcid":false,"given":"Claudia","family":"Bressan","sequence":"additional","affiliation":[{"name":"Catalonian Anti\u2010Doping Laboratory Hospital del Mar Institute for Medical Research  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1413-8890","authenticated-orcid":false,"given":"Rosa","family":"Ventura","sequence":"additional","affiliation":[{"name":"Catalonian Anti\u2010Doping Laboratory Hospital del Mar Institute for Medical Research  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4807-2826","authenticated-orcid":false,"given":"Alan","family":"\u00c1lvarez\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Organic Chemistry Section, Chemical Sciences Department, Laboratory of Medicinal Green Chemistry, Faculty of Higher Studies Cuautitlan National Autonomous University of Mexico  Cuautitl\u00e1n Izcalli Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4951-0689","authenticated-orcid":false,"given":"Enrique","family":"Cardenas\u2010Yong","sequence":"additional","affiliation":[{"name":"Organic Chemistry Section, Chemical Sciences Department, Laboratory of Medicinal Green Chemistry, Faculty of Higher Studies Cuautitlan National Autonomous University of Mexico  Cuautitl\u00e1n Izcalli Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2934-8698","authenticated-orcid":false,"given":"Benjam\u00edn","family":"Velasco\u2010Bejarano","sequence":"additional","affiliation":[{"name":"Organic Chemistry Section, Chemical Sciences Department, Laboratory of Medicinal Green Chemistry, Faculty of Higher Studies Cuautitlan National Autonomous University of Mexico  Cuautitl\u00e1n Izcalli Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-6844-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000028393"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/pl00000897"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0731\u20107085(03)00073\u20106"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.19052\/mv.3618"},{"issue":"4","key":"e_1_2_8_7_1","first-page":"369","article-title":"Dopaje y Control Antidopaje en el Deporte","volume":"83","author":"Mart\u00ednez\u2010Vargas A. 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