{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:51:39Z","timestamp":1790013099462,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Erythropoietin receptor agonists (ERAs), including continuous erythropoietin receptor activators (CERAs), are potent blood doping substances used to enhance endurance performance by stimulating erythropoiesis. While traditionally detected through direct analysis of urine or serum samples using sarcosyl\u2010polyacrylamide gel electrophoresis (SAR\u2010PAGE) and western blotting, the slow urinary elimination of third\u2010generation ERAs like CERA has shifted anti\u2010doping strategies toward serum\u2010based detection. This study compared the detectability and stability of CERA in urine and serum matrices and evaluated the added value of combining direct detection with hematological profiling. Using samples from a controlled CERA administration study and an authentic case example, we assessed CERA detection in serum, urine, and simulated dried blood spot (DBS) matrices (Tasso\u2010M20). Additionally, we conducted stability experiments by incubating spiked matrices at 37\u00b0C for up to 72\u2009h. Our results confirmed the superior stability and consistent detectability of CERA in serum and DBS compared with urine. Moreover, hematological alterations such as increased reticulocytes percentage flagged by the Athlete Biological Passport (ABP) supported targeted serum testing, leading to the successful detection of CERA. These findings highlight the importance of systematic blood collection for both direct and indirect detection strategies. Furthermore, DBS samples showed promising analytical performance and resistance to elevated temperature, suggesting their utility as minimally invasive alternatives in anti\u2010doping programs. Overall, our study reinforces the relevance of blood matrices in the detection of CERA and advocates for the broader integration of blood\u2010based strategies in targeting doping practices with ERAs.<\/jats:p>","DOI":"10.1002\/dta.3960","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T06:33:18Z","timestamp":1761114798000},"page":"16-23","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["CERA Detection and Stability in Blood Versus Urine"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0388-1352","authenticated-orcid":false,"given":"Olivier","family":"Salamin","sequence":"first","affiliation":[{"name":"Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne and Geneva Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9phine","family":"Chappuis","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne and Geneva Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lasse\u00a0V.","family":"B\u00e6kken","sequence":"additional","affiliation":[{"name":"Nordic Athlete Passport Management Unit, Norwegian Doping Control Laboratory, Department of Pharmacology Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiia","family":"Kuuranne","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne and Geneva Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Leuenberger","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne and Geneva Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.01.033"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.97"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2185"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.406"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00194\u2010021\u201000456\u2010x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fspor.2019.00030"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3596"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3831"},{"key":"e_1_2_8_10_1","unstructured":"World Anti Doping Agency (WADA) \u201cTechnical Document for the Harmonization of Analysis and Reporting of Erythropoietin (EPO)\u2010Receptor Agonists (ERAs) and Transforming Growth Factor\u2010Beta (TGF\u2010b) Signalling Inhibitors by Polyacrylamide Gel Electrophoretic (PAGE) Analytical Methods \u201d (TD2024EPO). World Anti Doping Agency 2024 accessed August 5 2025 https:\/\/www.wada\u2010ama.org\/en\/resources\/lab\u2010documents\/td2024epo."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2703"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.06.038"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3142"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2418"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2863"},{"key":"e_1_2_8_16_1","unstructured":"Cyclingnews \u201cVuelta a Costa Rica Winner Among 12 Riders to Test Positive in 2017 edition|Cyclingnews.com \u201d Cyclingnews 2018 accessed August 5 2025 https:\/\/www.cyclingnews.com\/news\/vuelta\u2010a\u2010costa\u2010rica\u2010winner\u2010among\u201012\u2010riders\u2010to\u2010test\u2010positive\u2010in\u20102017\u2010edition\/."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.270"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2021-0252"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1932"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.006809"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2011.07.054"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3736"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2011.06.008"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12010120"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio\u20102023\u20100118"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3254"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3738"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3960","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dta.3960","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T12:31:13Z","timestamp":1768307473000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.3960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,21]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/dta.3960"],"URL":"https:\/\/doi.org\/10.1002\/dta.3960","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,21]]},"assertion":[{"value":"2025-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}