{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T14:59:29Z","timestamp":1773673169249,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T00:00:00Z","timestamp":1333065600000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>We describe a rapid resolution liquid chromatography (RRLC) method for analyzing atropine sulfate, its degradation products (tropic acid, apoatropine, atropic acid) and other components (e.g. phenol, methylparaben) in injectable medicines that are used by the German armed forces in emergency situations. Chromatography is performed using an acetonitrile\/phosphate buffer gradient (pH\u2009=\u20091.0) and an RP 18 column (50 x 4.6\u2009mm, 1.8\u2009\u00b5m) with the detection wavelength set at 220\u2009nm. The concentration of the active ingredient (atropine sulfate) in the tested products ranges from about 1\u2009mg\u2022ml<jats:sup>\u20101<\/jats:sup> to 10\u2009mg\u2022ml<jats:sup>\u20101<\/jats:sup>. The concentrations of the detected degradation products range from 0.2% to 4.7% (tropic acid) in relation to the active pharmaceutical ingredient (API). Using shorter separation columns and smaller particle sizes of the stationary phase improved analysis time from 40 to 10\u2009min and reduced the consumption of solvents by approximately 75%. Owing to the pressure conditions (&lt; 200\u2009bar), UHPLC (ultra high performance liquid chromatography) systems are not needed. Comparison of the atropine and tropic acid results obtained with the previously used HPLC (high performance liquid chromatography) method of the MAH (marketing authorization holder) show that there is no indication of a significant difference between the two methods. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.402","type":"journal-article","created":{"date-parts":[[2012,3,31]],"date-time":"2012-03-31T21:25:22Z","timestamp":1333229122000},"page":"222-228","source":"Crossref","is-referenced-by-count":6,"title":["Rapid resolution liquid chromatography for monitoring the quality of stockpiled atropine preparations for injection"],"prefix":"10.1002","volume":"4","author":[{"given":"Thomas","family":"Zimmermann","sequence":"first","affiliation":[{"name":"Zentrales Institut des Sanitaetsdienstes der Bundeswehr Muenchen  Garching\u2010Hochbrueck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Dimmel","sequence":"additional","affiliation":[{"name":"Zentrales Institut des Sanitaetsdienstes der Bundeswehr Muenchen  Garching\u2010Hochbrueck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"J\u00fcttemeyer","sequence":"additional","affiliation":[{"name":"Sanitaetsamt der Bundeswehr  Muenchen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietmar","family":"Springer","sequence":"additional","affiliation":[{"name":"Zentrales Institut des Sanitaetsdienstes der Bundeswehr Muenchen  Garching\u2010Hochbrueck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Loch","sequence":"additional","affiliation":[{"name":"Zentrales Institut des Sanitaetsdienstes der Bundeswehr Muenchen  Garching\u2010Hochbrueck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,30]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"International programme on chemical safety evaluation; antidotes for poisoning by organophosphorus pesticides.Monograph on Atropine. Available at:http:\/\/www.inchem.org\/documents\/antidote\/antidote\/atropine.htm"},{"key":"e_1_2_6_3_1","unstructured":"OMCL Network of the Council of Europe.Stockpiling of Medicines \u2013 Monitoring \u2013 General Considerations.PA\/PH\/OMCL (09) 05 R 2009."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1197\/j.aem.2003.06.014"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20636"},{"key":"e_1_2_6_6_1","unstructured":"Atropinsulfat (Atropini sulfas) in Europ\u00e4isches Arzneibuch. Deutscher Apothekerverlag Stuttgart 1. und 3. Nachtrag 6.1\/0068 und 6.3\/0068 2009."},{"key":"e_1_2_6_7_1","unstructured":"Atropinsulfat (Atropini sulfas) in Europ\u00e4isches Arzneibuch. Deutscher Apothekerverlag Stuttgart 6.0\/0068 6. Ausgabe 2008."},{"key":"e_1_2_6_8_1","unstructured":"Atropin (Atropinum) in Europ\u00e4isches Arzneibuch. Deutscher Apothekerverlag Stuttgart 6.0\/2056 6. Ausgabe 2008."},{"key":"e_1_2_6_9_1","unstructured":"Atropinsulfat\u2010L\u00f6sungen 0 025% bis 1%. inStandardzulassungen f\u00fcr Fertigarzneimittel Deutscher Apothekerverlag Stuttgart 15. Erg.\u2010Lfg. Stand Juli2003."},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.05.088"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-3209-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-010-1524-y"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200800078"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.03.007"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2006.04.015"},{"key":"e_1_2_6_16_1","unstructured":"International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use.ICH Harmonised Tripartite Guideline Stability Testing of New Drug Substances and Products. Q1A(R2). February2003."},{"key":"e_1_2_6_17_1","volume-title":"Validierung in der Analytik","author":"Kromidas S.","year":"2007"},{"key":"e_1_2_6_18_1","unstructured":"DIN 32645 (Nachweis\u2010 Erfassungs\u2010 und Bestimmungsgrenze \u2013 Ermittlung unter Wiederholbedingungen Begriffe Verfahren Auswertung) November2008."},{"key":"e_1_2_6_19_1","unstructured":"EDQM. Qualification of HPLC Equipment (in Qualification of Equipment Annex 1).PA\/PH\/OMCL (07) 17 DEF February2007."},{"key":"e_1_2_6_20_1","volume-title":"Atropinsulfat (","author":"M\u00fcller A.","year":"2009"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdta.402","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T17:41:12Z","timestamp":1760377272000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":19,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/dta.402"],"URL":"https:\/\/doi.org\/10.1002\/dta.402","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3]]}}}