{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T10:28:40Z","timestamp":1775039320769,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001962","name":"World Anti-Doping Agency","doi-asserted-by":"publisher","award":["ISF15E10YP"],"award-info":[{"award-number":["ISF15E10YP"]}],"id":[{"id":"10.13039\/501100001962","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recombinant human erythropoietin (rHuEPO) is often misused in endurance sports due to its potent erythropoietic effects. While transcriptomic biomarkers hold promise for detecting rHuEPO use beyond conventional testing windows, many proposed gene markers may also respond to physiological stimuli such as exercise or altitude. This study compared 153 previously reported rHuEPO\u2010responsive genes in whole blood with transcripts identified during exercise (GEPREP database) and high\u2010altitude exposure (four independent studies). For the exercise dataset, gene\u2010level statistical outputs were obtained directly from the GEPREP database, while biological relevance was calculated using Cohen's\n                    <jats:italic>d<\/jats:italic>\n                    . Analyses of altitude and rHuEPO datasets followed the original statistical procedures described in each study. Among the 153 rHuEPO\u2010responsive genes, 94 overlapped with altitude and 34 with exercise. However, 50 genes remained unaffected by either exercise or altitude stimuli. Enriched in post\u2010translational regulation and intracellular transport pathways, these genes represent promising candidate transcriptomic markers of rHuEPO administration. This work provides a refined gene panel that reduces the likelihood of false positives and requires further experimental validation before integration into RNA\u2010based detection tests.\n                  <\/jats:p>","DOI":"10.1002\/dta.70040","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:13:14Z","timestamp":1773004394000},"page":"581-602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating Transcriptomic Biomarkers for rHuEPO Detection: Assessing the Impact of Exercise and Altitude Exposure"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9495-0136","authenticated-orcid":false,"given":"Daria","family":"Obratov","sequence":"first","affiliation":[{"name":"Department of Sports and Health Sciences Hong Kong Baptist University  Kowloon Tong Hong Kong SAR China"},{"name":"Centre for Exercise Science and Medicine (CESAME) Hong Kong Baptist University  Kowloon Tong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3363-7975","authenticated-orcid":false,"given":"Shaun","family":"Sutehall","sequence":"additional","affiliation":[{"name":"Research Institute for Sport and Exercise Sciences Liverpool John Moores University  Liverpool UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6501-863X","authenticated-orcid":false,"given":"Longhua","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Exercise and Health Shanghai University of Sport  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-9008","authenticated-orcid":false,"given":"Zhao","family":"Zhongying","sequence":"additional","affiliation":[{"name":"Centre for Exercise Science and Medicine (CESAME) Hong Kong Baptist University  Kowloon Tong Hong Kong SAR China"},{"name":"Department of Biology, Faculty of Science Hong Kong Baptist University  Kowloon Tong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6210-2449","authenticated-orcid":false,"given":"Yannis","family":"Pitsiladis","sequence":"additional","affiliation":[{"name":"Centre for Exercise Science and Medicine (CESAME) Hong Kong Baptist University  Kowloon Tong Hong Kong SAR China"},{"name":"Department of Biology, Faculty of Science Hong Kong Baptist University  Kowloon Tong Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01553038"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1972.33.2.175"},{"issue":"5","key":"e_1_2_11_4_1","first-page":"521","article-title":"Erythropoietin, the Biology of Erythropoiesis and Epoetin Alfa. An Overview","volume":"46","author":"Bieber E.","year":"2001","journal-title":"Journal of Reproductive Medicine"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198701083160203"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000356193"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421\u2010007\u20100522\u20108"},{"key":"e_1_2_11_8_1","unstructured":"World Anti\u2010Doping Agency \u201cAthlete Biological Passport (ABP) Operating Guidelines \u201d (2023) https:\/\/www.wada\u2010ama.org\/en\/resources\/world\u2010anti\u2010dopingprogram\/athlete\u2010biological\u2010passport\u2010abp\u2010operating\u2010guidelines#resource\u2010download."},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/lpr\/mgm042"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40798\u2010021\u201000356\u20100"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2011.162271"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00108.2015"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.911861"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010017\u20104191\u20107"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201000608\u20109"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/JSM.0000000000001046"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP282772"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.14429\/dlsj.9.19454"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111213"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jshs.2024.100992"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_11_22_1","unstructured":"M.Carlson H.Pages andN.Li. \u201corg.Hs.eg.db: Genome Wide Annotation for Human. 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