{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T10:28:45Z","timestamp":1775039325513,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100013935","name":"Partnership for Clean Competition","doi-asserted-by":"publisher","award":["2018 R318"],"award-info":[{"award-number":["2018 R318"]}],"id":[{"id":"10.13039\/100013935","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201020\u2010CE14\u20100043\u201001"],"award-info":[{"award-number":["ANR\u201020\u2010CE14\u20100043\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201024\u2010CE14\u20100739\u201005"],"award-info":[{"award-number":["ANR\u201024\u2010CE14\u20100739\u201005"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013935","name":"Partnership for Clean Competition","doi-asserted-by":"publisher","award":["R318"],"award-info":[{"award-number":["R318"]}],"id":[{"id":"10.13039\/100013935","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Since the implementation of the first erythropoietin (EPO) detection method to fight the use of recombinant EPO (rEPO) for doping, the techniques used and identification criteria have evolved. The first technique involved iso\u2010electric focusing (IEF\u2010PAGE) to separate EPO isoforms by their charges. It can still be used for initial testing but is now largely replaced by electrophoretic separation based on molecular weight (SDS\u2010PAGE or SAR\u2010PAGE) to conclude decisively on the presence of rEPO. Recently, different mutations in the noncoding region of the\n                    <jats:italic>EPO<\/jats:italic>\n                    gene were identified in patients with familial erythrocytosis. Their blood EPO profiles after IEF\u2010PAGE were very basic and in the same area as rEPO. To avoid any possible misinterpretation in an antidoping context, the goal of this work was to characterize urine and blood EPO profiles from these patients using all methods authorized in antidoping laboratories (IEF\u2010PAGE, SDS\u2010PAGE, and SAR\u2010PAGE) and to compare them with wild\u2010type (WT) EPO and with profiles obtained after the administration of rEPO Eprex. Results showed that SDS\u2010PAGE and SAR\u2010PAGE profiles were not modified in the patients compared with WT EPO and could not be mistaken for the presence of rEPO. In contrast, the IEF\u2010PAGE profiles in urine were slightly more acidic than in blood and closer to the rEPO profile. However, they did not meet the identification criteria used when this technique was the only authorized method. It is highly unlikely that these\n                    <jats:italic>EPO<\/jats:italic>\n                    mutations, only identified in erythrocytosis patients yet, could lead to an adverse analytical finding.\n                  <\/jats:p>","DOI":"10.1002\/dta.70049","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T01:24:56Z","timestamp":1771550696000},"page":"537-542","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of a Distinct Erythropoietin (EPO) Profile After Isoelectric Focusing in Patients With Familial Erythrocytosis"],"prefix":"10.1002","volume":"18","author":[{"given":"Laurent","family":"Martin","sequence":"first","affiliation":[{"name":"Laboratoire Antidopage Fran\u00e7ais (LADF) Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5439-8726","authenticated-orcid":false,"given":"Salam","family":"Idriss","sequence":"additional","affiliation":[{"name":"l'institut du thorax Nantes Universit\u00e9, CNRS, INSERM  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nada","family":"Maaziz","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, P\u00f4le Biologie CHU de Dijon  Dijon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soizic","family":"Huerre","sequence":"additional","affiliation":[{"name":"Laboratoire Antidopage Fran\u00e7ais (LADF) Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamine\u00a0Redouane","family":"Mekacher","sequence":"additional","affiliation":[{"name":"Laboratoire de Toxicologie Centre Hospitalier Universitaire Tizi\u2010Ouzou  Tizi\u2010Ouzou Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kahina","family":"Lahmek","sequence":"additional","affiliation":[{"name":"Laboratoire de Toxicologie Centre Hospitalier Universitaire Tizi\u2010Ouzou  Tizi\u2010Ouzou Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Girodon","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Biologique, P\u00f4le Biologie CHU de Dijon  Dijon France"},{"name":"Laboratoire d'Excellence GR\u2010Ex  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8206-8329","authenticated-orcid":false,"given":"Betty","family":"Gardie","sequence":"additional","affiliation":[{"name":"l'institut du thorax Nantes Universit\u00e9, CNRS, INSERM  Nantes France"},{"name":"Laboratoire d'Excellence GR\u2010Ex  Paris France"},{"name":"EPHE PSL Universit\u00e9  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Ericsson","sequence":"additional","affiliation":[{"name":"Laboratoire Antidopage Fran\u00e7ais (LADF) Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2670-6939","authenticated-orcid":false,"given":"Alexandre","family":"Marchand","sequence":"additional","affiliation":[{"name":"Laboratoire Antidopage Fran\u00e7ais (LADF) Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"640","article-title":"Liver as the Primary Site of Erythropoietin Formation in the Fetus","volume":"89","author":"Zanjani E. D.","year":"1977","journal-title":"Journal of Laboratory and Clinical Medicine"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3902"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110133"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.08.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0265022"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V73.1.84.84"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/35015164"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00407-4"},{"key":"e_1_2_9_10_1","volume-title":"Recent Advances in Doping Analysis","author":"Lasne F.","year":"2005"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2007.04.002"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/JSM.0b013e3181a9483e"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2601"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-8793-1_14"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3200"},{"key":"e_1_2_9_16_1","unstructured":"Harmonization of Analysis and Reporting of Erythropoietin (EPO) Receptor Agonists (ERAs) and Transforming Growth Factor\u2010Beta (TGF\u2010\u03b2) Signalling Inhibitors by Polyacrylamide Gel Electrophoretic (PAGE) Analytical Methods TD2024EPO v1.0(2024) https:\/\/www.wada\u2010ama.org\/en\/resources\/lab\u2010documents\/td2024epo."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2414954"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2904"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113750"},{"key":"e_1_2_9_20_1","unstructured":"Harmonization of Analysis and Reporting of Recombinant Erythropoietins (i.e. epoetins) and Analogues (e.g. Darbepoetin Pegserpoetin Peginesatide EPO\u2010Fc) by Electrophoretic Techniques TD2013EPO v1.0(2012) https:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2022\u201001\/WADA\u2010TD2013EPO\u2010Harmonization\u2010Analysis\u2010of\u2010Recombinant\u2010Erythropoietins\u2010EN.pdf."},{"key":"e_1_2_9_21_1","unstructured":"Harmonization of the Method for the Identification of Recombinant Erythropoietins (i.e. Epoetins) and Analogues (e.g. Darbepoetin and Methoxypolyethylene Glycol\u2010Epoetin Beta) TD2009EPO v2.0(2009) https:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2022\u201001\/WADA_TD2009EPO_Harmonization_of_Method_Identificiation_Recombinant_Erythropoietins_Analogues_EN.pdf."}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.70049","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dta.70049","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.70049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T08:26:16Z","timestamp":1775031976000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.70049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,19]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/dta.70049"],"URL":"https:\/\/doi.org\/10.1002\/dta.70049","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,19]]},"assertion":[{"value":"2025-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}