{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T08:38:23Z","timestamp":1777365503308,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A subject, admitted to an addiction treatment unit, was occupationally exposed to solvent mixtures and paint thinners containing aliphatic alcohols, while being a coachbuilder. He repeatedly tested positive for ethyl glucuronide (EtG) in weekly urine immunoassays performed in a hospital laboratory. However, he denied any alcohol consumption. Therefore, presumptive positive urine samples, as well as chest and pubic hair samples, were submitted to targeted liquid chromatography\u2013high\u2010resolution accurate mass\u2013Orbitrap\u2013mass spectrometry (LC\u2010HRAM\u2010Orbitrap\u2010MS) analysis, after derivatization of analytes with N\u2010(3\u2010dimethylaminopropyl)\u2010N\u2032\u2010ethylcarbodiimide (EDC). MS acquisition was carried out in full scan positive\u2010ion mode, followed by data\u2010dependent MS\n                    <jats:sup>2<\/jats:sup>\n                    and parallel reaction monitoring confirmation modes, according to an inclusion list of accurate mass values calculated from the elemental composition of MH\n                    <jats:sup>+<\/jats:sup>\n                    ion species of several derivatized EtG homologs. All urine samples turned out to be EtG negative. Instead, methyl, propyl, butyl, and hexyl glucuronides were detected at varying levels depending on the exposure and\/or inhalation conditions (product types, amounts, etc.), and urine sampling times. As expected, chest hair and pubic hair were EtG negative too, whereas the same other alcohol glucuronides were detected in both matrices, with higher abundances in pubic hair. Cross\u2010reaction of EtG homologs, in this case due to exposure to chemicals containing several aliphatic alcohols, explains the false\u2010positive urine immunoassay results. Furthermore, LC\u2010HRAM\u2010Orbitrap\u2010MS analyses show that EtG homologs, like EtG, can be incorporated and detected in a variety of hair samples.\n                  <\/jats:p>","DOI":"10.1002\/dta.70064","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T22:20:51Z","timestamp":1774390851000},"page":"699-708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["False\u2010Positive EtG Immunoassay Screening After Exposure to Aliphatic Alcohols. LC\u2010HRAM\u2010Orbitrap\u2010MS Detection of C1\u2013C6 EtG Homologs in Urine, Chest, and Pubic Hair Samples"],"prefix":"10.1002","volume":"18","author":[{"given":"Camilla","family":"Vigato","sequence":"first","affiliation":[{"name":"Laboratory of Clinical and Forensic Toxicology, DMPO Department, AULSS 3  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavio","family":"Zancanaro","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical and Forensic Toxicology, DMPO Department, AULSS 3  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Novella","family":"Ghezzo","sequence":"additional","affiliation":[{"name":"Addiction Treatment Department, AULSS 3  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6545-4109","authenticated-orcid":false,"given":"Giampietro","family":"Frison","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical and Forensic Toxicology, DMPO Department, AULSS 3  Venice Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1359\u20106357.2003.00588.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20102999(67)90067\u20102"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/19.2.91"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agy064"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2007.01.005"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2009.03.017"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2015.10.017"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/26.5.262"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agaa113"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2019.05.004"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2008.12.005"},{"key":"e_1_2_10_13_1","volume-title":"2019 Consensus for the Use of Alcohol Markers in Hair for Supporting the Assessment of Abstinence and Chronic Alcohol Consumption","year":"2019"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2011.011115"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2015.10.004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/00952990.2015.1011743"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.70000"},{"issue":"3","key":"e_1_2_10_18_1","article-title":"The Metabolism of Aliphatic Alcohols; Glucuronide Formation","volume":"49","author":"Kamil I. 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