{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:31:10Z","timestamp":1791725470483,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diphenhydramine and cyclizine are over\u2010the\u2010counter (in many countries) antihistamines that have considerable sedative effects. Therefore, they can be used in drug\u2010facilitated crimes to incapacitate victims. There is a knowledge gap regarding detection possibilities and concentration levels of these antihistamines in hair. This study aimed to investigate whether a single dose of diphenhydramine and cyclizine could be quantified in head hair, and for how long. A single\u2010dose study was conducted in which 12 adult volunteers ingested a single dose of diphenhydramine (25\u2009mg) and cyclizine (44\u2009mg). Hair samples were collected before drug intake and at 1, 3, 5, and if possible, 12\u2009months after intake. To quantify a single dose of antihistamines in head hair, a validated LC\u2013MS\/MS method with a lower limit of quantification of 1\u2009pg\/mg was applied. Both antihistamines and their main metabolites,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010demethyldiphenhydramine and norcyclizine, were detected in all hair samples collected up to 5\u2009months after intake. Twelve months after intake, diphenhydramine was quantifiable in 25% and cyclizine in 62.5% of available hair samples. The concentrations found in hair within 1\u2009year after intake ranged from 0 to 610\u2009pg\/mg for diphenhydramine and from 0 to 590\u2009pg\/mg for cyclizine. The median decrease in concentration over 2, 4, and 11\u2009months was 43%, 70%, and 100% for diphenhydramine and 40%, 64%, and 98% for cyclizine. The data obtained provided indicative concentration ranges for a single oral dose of diphenhydramine and cyclizine in hair, thereby improving the interpretation of hair analyses in forensic casework.\n                  <\/jats:p>","DOI":"10.1002\/dta.70084","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T02:52:22Z","timestamp":1777863142000},"page":"877-892","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Segmental Hair Analysis of Diphenhydramine and Cyclizine Following a Single Dose"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9669-2829","authenticated-orcid":false,"given":"Jan","family":"B\u00edlek","sequence":"first","affiliation":[{"name":"Section of Forensic Chemistry, Department of Forensic Medicine, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen 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