{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T17:53:22Z","timestamp":1785779602300,"version":"3.56.0"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dried blood spot (DBS) sampling offers logistical advantages, but it remains unexplored whether analytical results are comparable across DBS sampling methods. This study investigated whether venous or capillary blood spotted on cellulose cards or collected with the Tasso\u2010M20 device provides comparable results for the anti\u2010doping RNA\u2010based biomarkers of erythropoiesis, 5\u2010aminolevulinic acid synthase (\n                    <jats:italic>ALAS2<\/jats:italic>\n                    ), and carbonic anhydrase 1 (\n                    <jats:italic>CA1<\/jats:italic>\n                    ). In the population of Swiss athletes (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912), DBS samples (venous\u2010cellulose and Tasso\u2010M20) were collected in routine anti\u2010doping procedures. In healthy volunteers (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912), DBS samples (venous\u2010cellulose, capillary\u2010cellulose and Tasso\u2010M20) were collected at baseline, after 1\u2009week and 10\u2009days post a 3\u2010week recombinant human erythropoietin (rEPO) administration study.\n                    <jats:italic>ALAS2<\/jats:italic>\n                    and\n                    <jats:italic>CA1<\/jats:italic>\n                    mRNA were quantified using RT\u2010qPCR, and agreement between matrices was assessed via Passing\u2013Bablok regression.\n                    <jats:italic>ALAS2<\/jats:italic>\n                    and\n                    <jats:italic>CA1<\/jats:italic>\n                    expression showed strong linear agreement (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009\u2265\u20090.96) across matrices. Passing\u2013Bablok regression indicated no constant or proportional bias for\n                    <jats:italic>ALAS2<\/jats:italic>\n                    across all comparisons. For\n                    <jats:italic>CA1<\/jats:italic>\n                    , no bias existed between venous\u2010cellulose and Tasso\u2010M20 in the athlete population, whereas a proportional bias of ~9%\u201322% was observed when comparing Tasso\u2010M20 DBS with venous\u2010 or capillary\u2010cellulose DBS in the rEPO study. No significant differences in relative RNA expression were observed across matrices at any timepoint in the administration study.\n                    <jats:italic>ALAS2<\/jats:italic>\n                    and\n                    <jats:italic>CA1<\/jats:italic>\n                    are reliably quantified across venous\u2010 and capillary\u2010cellulose\u2010based DBS and Tasso\u2010M20 DBS samples. Strong agreement and minimal bias, with the modest\n                    <jats:italic>CA1<\/jats:italic>\n                    bias being small relative to expected biological or treatment\u2010induced variability, support their use for longitudinal monitoring, enabling less invasive, flexible, and decentralized sampling in research and anti\u2010doping.\n                  <\/jats:p>","DOI":"10.1002\/dta.70091","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:33:32Z","timestamp":1779323612000},"page":"959-966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Dried Blood Spot Sampling Methods for RNA\u2010Based Biomarker Measurement in Anti\u2010Doping"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9698-9188","authenticated-orcid":false,"given":"Jacob","family":"Bejder","sequence":"first","affiliation":[{"name":"Department of Nutrition, Exercise and Sports (NEXS) University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corentin","family":"Schlechten","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses University Center of Legal Medicine, Lausanne and Geneva, Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mumenthaler","sequence":"additional","affiliation":[{"name":"Swiss Sport Integrity  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0388-1352","authenticated-orcid":false,"given":"Olivier","family":"Salamin","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses University Center of Legal Medicine, Lausanne and Geneva, Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1638-6182","authenticated-orcid":false,"given":"Tiia","family":"Kuuranne","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses University Center of Legal Medicine, Lausanne and Geneva, Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas\u00a0Christian","family":"Bonne","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports (NEXS) University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Graae","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports (NEXS) University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Anesthesia Herlev\/Gentofte Hospital  Herlev Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai\u00a0B.","family":"Nordsborg","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports (NEXS) University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7106-6304","authenticated-orcid":false,"given":"Nicolas","family":"Leuenberger","sequence":"additional","affiliation":[{"name":"Swiss Laboratory for Doping Analyses University Center of Legal Medicine, Lausanne and Geneva, Lausanne University Hospital and University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3665"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2240"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3939"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3407"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio\u20102023\u20100251"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2019.306829"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3870"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3135"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3647"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3960"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3889"},{"key":"e_1_2_10_13_1","unstructured":"\u201cTD2026DBS 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