{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:26:52Z","timestamp":1788276412156,"version":"build-2803163510"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Homologous blood transfusion (HBT) doping, using donor red blood cells (RBCs) to enhance oxygen capacity, remains a persistent challenge for doping control laboratories, especially in specific populations. Current RBC antigen\u2010based flow cytometric detection (double population, DP) has limited sensitivity in genetically homogeneous populations due to common phenotypic overlap. This study aimed to enhance HBT detection by optimizing population\u2010specific RBC antigen panels and incorporating complementary platelet\u2010derived human leukocyte antigen (HLA) markers. Based on antigen\u2010negative frequency, M, N, and Le\n                    <jats:sup>b<\/jats:sup>\n                    antigens were evaluated as candidate markers for improved discrimination in the Japanese population. In simulated HBT samples with a 5% mixing ratio, N antigen showed a clearly separated bimodal distribution, indicating strong potential for DP detection. Although Le\n                    <jats:sup>b<\/jats:sup>\n                    antigen showed unclear DP, M antigen displayed uniformly high expression, useful for distinguishing expressing populations. In addition, platelet HLA analysis, especially targeting HLA\u2010A24, enabled detection of donor\u2010derived components even with identical RBC antigen phenotypes. Mixed samples containing as low as 1%\u20135% donor cells produced visually detectable DP signals in the platelet gate. In conclusion, integrating a race\u2010tailored RBC antigen panel with complementary platelet HLA monitoring may provide a potentially improved framework for HBT detection, particularly in ethnically homogeneous populations like Japan. The optimal antigen panel is highly population\u2010specific due to genetic variations, requiring adaptation for other diverse ethnic groups. Future cohort studies are warranted to validate this dual approach and refine detection sensitivity.\n                  <\/jats:p>","DOI":"10.1002\/dta.70108","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:25:49Z","timestamp":1782282349000},"page":"1186-1197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Potential of Population\u2010Optimized Red Blood Cell Antigens and Platelet HLA Typing for Homologous Blood Transfusion Detection in Japanese Athletes"],"prefix":"10.1002","volume":"18","author":[{"given":"Atsushi","family":"Momobayashi","sequence":"first","affiliation":[{"name":"Anti\u2010Doping Laboratory LSI Medience Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3614-4121","authenticated-orcid":false,"given":"Masato","family":"Okano","sequence":"additional","affiliation":[{"name":"Anti\u2010Doping Laboratory LSI Medience Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"World Anti\u2010Doping Agency \u201cWorld Anti\u2010Doping Code. International Standard. Prohibited List 2026 Montreal 2025 \u201d accessed January 25 2026 https:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2025\u201009\/2026list_en_final_clean_september_2025.pdf."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2008.04.007"},{"issue":"11","key":"e_1_2_10_4_1","first-page":"1284","article-title":"Proof of Homologous Blood Transfusion Through Quantification of Blood Group Antigens","volume":"88","author":"Nelson M.","year":"2003","journal-title":"Haematologica"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102007\u2010965076"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fspor.2022.808449"},{"key":"e_1_2_10_7_1","unstructured":"World Anti\u2010Doping Agency \u201cDetection of Homologous Blood Transfusion (HBT) by Flow Cytometry WADA Technical Document\u2013TD2026HBT Montreal \u201d (2025) accessed January 24 2026 https:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2025\u201009\/td2026hbt_final_eng.pdf."},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3381"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3402"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.5348\/100053Z02MW2020RA"},{"key":"e_1_2_10_11_1","unstructured":"Japanese Red Cross Society \u201cBlood Transfusion Information [in Japanese] \u201d 1511\u2013145 (2015) accessed January 24 2026 https:\/\/www.jrc.or.jp\/mr\/relate\/info\/pdf\/yuketsuj_1511\u2010145.pdf."},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2008.04.001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423\u20100410.1987.tb04908.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537\u20102995.1990.30290162896.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20100502(01)00070\u20102"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/176060"},{"issue":"4","key":"e_1_2_10_17_1","first-page":"390","article-title":"HLA Antigens in Japanese Populations","volume":"28","author":"Yasuda N.","year":"1976","journal-title":"American Journal of Human Genetics"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12536"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0195778"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13133267"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.5001\/omj.2019.92"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3709"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.70108","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dta.70108","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.70108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:07:56Z","timestamp":1788275276000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.70108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":21,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/dta.70108"],"URL":"https:\/\/doi.org\/10.1002\/dta.70108","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]},"assertion":[{"value":"2026-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}