{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T02:02:48Z","timestamp":1789956168022,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Doping control samples showing elevated urinary concentrations and concentration ratios of testosterone or testosterone metabolites are forwarded to isotope ratio mass spectrometry\u2010based determinations in order to differentiate between naturally elevated concentrations and doping offenses. Routine sample preparation protocols commonly encompass liquid\u2013liquid and solid\u2010phase extraction steps and enzymatic deconjugation of steroid glucuronides. In order to separate all steroids of interest from matrix components and to obtain sufficiently clean urinary extracts, high performance liquid chromatography (HPLC) with fraction collection is a frequently employed method of choice. This preparation step is time consuming as each HPLC run requires approx. 45\u2009min, and the evaporation of collected fractions containing water and acetonitrile necessitates up to 60\u2009min. Supercritical fluid chromatography employs supercritical carbon dioxide as eluent combined with other organic solvents as modifiers. The unique physiochemical properties of supercritical carbon dioxide enable the acceleration of the chromatographic separation of different compounds, and collected fractions only contain the modifier and additional methanol as make\u2010up solvent, which allows for rapid evaporation within 10\u2009min. Therefore, a method was developed and validated in\u2010line with current World Anti\u2010Doping Agency\u2010stipulated regulations encompassing testosterone, epitestosterone (E), dehydroepiandrosterone, androsterone, etiocholanolone, 5\u03b1\u2010 and 5\u03b2\u2010androstanediol as target analytes and cholesterol, pregnanediol, 16\u2010androstenol, and 11\u2010oxo\u2010etiocholanolone as endogenous reference compounds. Reference population data encompassed 40 female and 40 male routine doping control samples. Cholesterol as a new endogenous reference compound was carefully investigated. As proof\u2010of\u2010concept, samples derived from a testosterone\u2010gel administration trial were investigated, demonstrating an impact of the drug intervention on E.<\/jats:p>","DOI":"10.1002\/dta.70150","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T01:26:24Z","timestamp":1789953984000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Supercritical Fluid Chromatography for Expedited Sample Clean\u2010Up in Isotope Ratio Mass Spectrometry Including Cholesterol as New Endogenous Reference Compound"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7462-6693","authenticated-orcid":false,"given":"Thomas","family":"Piper","sequence":"first","affiliation":[{"name":"German Sport University Cologne Center for Preventive Doping Research \u2013 Institute of Biochemistry  K\u00f6ln Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2130-2493","authenticated-orcid":false,"given":"Jana","family":"Drewes","sequence":"additional","affiliation":[{"name":"University of M\u00fcnster Institute of Inorganic and Analytical Chemistry  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1535-6451","authenticated-orcid":false,"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[{"name":"German Sport University Cologne Center for Preventive Doping Research \u2013 Institute of Biochemistry  K\u00f6ln Germany"},{"name":"European Monitoring Center for Emerging Doping Agents (EuMoCEDA) Cologne\/Bonn  North Rhine\u2010Westphalia Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Analytical and Reporting Requirements for 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