{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:47:53Z","timestamp":1792043273976,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>CJC\u20101295 is a peptide\u2010based drug that stimulates the endogenous production of growth hormone (GH). Compared to other growth hormone releasing hormone (GHRH) analogues, CJC\u20101295 has an extended half\u2010life due to a drug affinity complex (DAC) that chemically crosslinks the peptide to plasma proteins containing free cysteine residues. As a result, CJC\u20101295 behaves more like a protein doping agent than a typical peptide\u2010based drug. Using monoclonal antibodies raised against the CJC\u20101295 peptide, an ELISA was developed for the detection of CJC\u20101295 protein conjugates in equine plasma at concentrations as low as 12.5\u2009pg\/mL. The effectiveness of the assay for controlling the illicit use of CJC\u20101295 was demonstrated using equine blood samples, obtained from thoroughbred racehorses, following subcutaneous administration of 10\u2009mg of CJC\u20101295.<\/jats:p>","DOI":"10.1002\/dta.70171","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:16:13Z","timestamp":1792041373000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of CJC\u20101295 in Equine Plasma by ELISA"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6657-337X","authenticated-orcid":false,"given":"Mark","family":"Timms","sequence":"first","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3442-8617","authenticated-orcid":false,"given":"Adam","family":"Cawley","sequence":"additional","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0423-6120","authenticated-orcid":false,"given":"Rohan","family":"Steel","sequence":"additional","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"3052","DOI":"10.1210\/en.2004-1286","article-title":"Human Growth Hormone\u2010Releasing Factor (hGRF)1\u201029\u2010Albumin Bioconjugates Activate the GRF Receptor on the Anterior Pituitary in Rats: Identification of CJC\u20101295 as a Long\u2010Lasting GRF Analog","volume":"146","author":"Jett\u00e9 L.","year":"2005","journal-title":"Endocrinology"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0006-291X(84)91647-4","article-title":"Super\u2010Active Analogs of Growth Hormone\u2010Releasing Factor (1\u201029)\u2010Amide","volume":"119","author":"Lance V. 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