{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T14:35:19Z","timestamp":1770474919384,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,30]],"date-time":"2002-01-30T00:00:00Z","timestamp":1012348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Receptor protein\u2010tyrosine phosphatase alpha (RPTP\u03b1) is highly expressed in the developing retina of different species, but little is known about its function there. Here, we report that injection of antisense morpholinos in zebrafish embryos reduced RPTP\u03b1 expression to almost nondetectable levels up to 3 days postfertilization (dpf). RPTP\u03b1 was detectable again from 4 dpf onward. RPTP\u03b1 knock\u2010down resulted in smaller eyes. Examination of sections of the retina at different developmental stages demonstrated that already at 28 hours postfertilization (hpf) fewer cells were present in the retina of RPTP\u03b1\u2010morpholino\u2013injected embryos. At 3 dpf, the layered organization of the retina was absent. In addition, the morphology and labeling with an axon specific antibody, acetylated tubulin, demonstrated that most cells appeared to be undifferentiated. Strikingly, at 5 dpf the lamination of the retina was partially restored, concomitant with re\u2010expression of RPTP\u03b1 protein. Although cells in the retina were now differentiated, the layering of the retina remained disrupted and significant gaps were observed in the amacrine cell layer. Therefore, knock\u2010down of RPTP\u03b1 protein provides evidence that RPTP\u03b1 is essential for normal retinal development. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10059","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T18:20:15Z","timestamp":1030299615000},"page":"292-297","source":"Crossref","is-referenced-by-count":19,"title":["Eye defects in receptor protein\u2010tyrosine phosphatase \u03b1 knock\u2010down zebrafish"],"prefix":"10.1002","volume":"223","author":[{"given":"Astrid M.","family":"Van Der Sar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danica","family":"\u017divkovi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeroen","family":"Den Hertog","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.6.849"},{"key":"e_1_2_5_3_1","first-page":"17","article-title":"La retine des vertebres","volume":"9","author":"Cajal SR","year":"1893","journal-title":"La Cellule"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-06-01892.1990"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00089-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(96)00561-8"},{"key":"e_1_2_5_7_1","volume-title":"The retina","author":"Dowling J","year":"1987"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90205-X"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9031"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00345-7"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80391-8"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00312-9"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1242\/dev.109.3.567","article-title":"Early onset of phenotype and cell patterning in the embryonic zebrafish retina","volume":"109","author":"Larison KD","year":"1990","journal-title":"Development"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199904)39:1<81::AID-NEU7>3.0.CO;2-K"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0340"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(00)01655-6"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.123.1.263"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00076-8"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/79951"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19971124)388:3<495::AID-CNE11>3.0.CO;2-L"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80238-3"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903590403"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903440404"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0145"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00179-3"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80234-6"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/328131a0"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90136-4"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00545-7"},{"key":"e_1_2_5_31_1","first-page":"785","article-title":"Pleiotropic effects of zebrafish protein\u2010tyrosine phosphatase\u20101B on early embryonic development","volume":"43","author":"van der Sar AM","year":"1999","journal-title":"Int J Dev Biol"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80139-7"},{"key":"e_1_2_5_33_1","doi-asserted-by":"crossref","unstructured":"VerbeekFJ den BroederMJ BoonPJ BuitendijkB van RaaijEJ DoerryE ZivkovicD.2000.A standard 3D digital atlas of zebrafish embryonic development for projection of experimental data. 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