{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T23:14:44Z","timestamp":1772838884465,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,4,11]],"date-time":"2002-04-11T00:00:00Z","timestamp":1018483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Differentially expressed nucleolar TGF\u2010\u03b21 target (DENTT) is a novel member of the TSPY\/TSPY\u2010L\/SET\/NAP\u20101 (TTSN) superfamily that we have previously identified in human lung cancer cells. Here, we have investigated the expression of this protein in the adult mouse. By Western analysis, DENTT is highly expressed in the pituitary gland and moderately in the adrenals, brain, testis, and ovary. Immunohistochemical staining analysis for DENTT showed differential cytoplasmic and nuclear staining patterns in several cell types. The pituitary gland showed the highest level of immunostaining for DENTT, with strong cytoplasmic immunoreactivity in the anterior lobe, moderate levels in the posterior lobe, and a few cells showing nuclear staining in the intermediate lobe. In contrast, the intermediate lobe of the pituitary showed intense cytoplasmic staining for TGF\u2010\u03b21. Nuclear and cytoplasmic staining for DENTT was present in the islets of Langerhans in the pancreas. Cytoplasmic staining for DENTT was particularly intense in the cortex of the adrenal gland, whereas the medulla showed weak nuclear staining. In the nervous system, the choroid plexus showed the highest immunoreactivity, with cortical motoneurons and Purkinje cells having relatively high levels of staining for DENTT as well. DENTT immunoreactivity was found in Leydig interstitial cells, Sertoli cells, and primary spermatocytes in the testis. In the female reproductive system, DENTT immunoreactivity was present in oocytes, thecal cells, and corpora lutea. The bronchial epithelium of the lung showed moderate levels of staining for DENTT localized to the cell nucleus. Additionally, three rodent pituitary cell lines (AtT20, GH3, and \u03b1T3\u20101, representing corticotropes, lactotropes, and gonadotropes, respectively) showed expression of DENTT. Addition of TGF\u2010\u03b21 or serum to AtT20 cells increased DENTT protein production by 4 hr and, after reaching maximal levels at 2.4\u2010fold above basal level by 8 hr, decreased, whereas no more than a 1.5\u2010fold increase in DENTT protein occurred in GH3 or \u03b1T3\u20101 cells. Transient transfection studies showed that ectopic DENTT expression significantly increased the level of p3TP\u2010Lux reporter transcription in AtT20 cells, but not in GH3 or \u03b1T3\u20101 cells. Interestingly, addition of TGF\u2010\u03b21 had no significant effect on the ability of DENTT expression to influence p3TP\u2010Lux reporter transcription in AtT20 cells. This report is the first detailed immunohistochemical examination of a member of the TTSN superfamily in the adult mouse. Expression of DENTT in endocrine tissues, nervous system, lung, oocytes, and thecal cells, in addition to the testis, suggests new roles for the TTSN superfamily. The differential patterns of expression of DENTT and TGF\u2010\u03b21 in some tissues, including the pituitary, suggest that other factors are likely to be regulators of DENTT besides TGF\u2010\u03b21. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10096","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T19:48:25Z","timestamp":1030304905000},"page":"186-199","source":"Crossref","is-referenced-by-count":15,"title":["Expression of differentially expressed nucleolar transforming growth factor\u2010\u03b21 target (DENTT) in adult mouse tissues"],"prefix":"10.1002","volume":"224","author":[{"given":"Alfredo","family":"Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent L.","family":"Ozbun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry","family":"Angdisen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia B.","family":"Jakowlew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,4,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90567-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1210\/endo.142.3.8028","article-title":"Regulation and actions of Smad7 in the modulation of activin, inhibin, and transforming growth\u2010factor\u2010\u03b2 signaling in anterior pituitary cells","volume":"142","author":"Bilezikjian LM","year":"2001","journal-title":"Endocrinology"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.10.2621"},{"key":"e_1_2_6_5_1","first-page":"2903","article-title":"Expression of TGF\u2010\u03b2 type II receptor mRNA in the CNS","volume":"7","author":"Bottner M","year":"1996","journal-title":"Mol Neurosci"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0752227.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.3.1444"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.6.3810"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend-3-12-1969"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.47.475"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1994.tb00988.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2000.6550255.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1490019"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"20980","DOI":"10.1016\/S0021-9258(19)36785-7","article-title":"Functional analysis of nucleosome assembly protein, NAP\u20101. 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