{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:12:07Z","timestamp":1762099927730,"version":"build-2065373602"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,5,15]],"date-time":"2002-05-15T00:00:00Z","timestamp":1021420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Submandibular gland (SMG) development involves branching morphogenesis of the salivary epithelium into the surrounding mesenchyme, accompanied by proliferation and differentiation of immature salivary cells along acinar and ductal cell lineages. During development, salivary cell sorting and cell\u2013cell adhesion are likely to be directed by cadherin adhesion receptors. We show that two classic cadherins, N\u2010 and E\u2010cadherin, participate in SMG development. Early in embryonic morphogenesis, both cadherins displayed diffuse staining with regionalized localization to cell\u2013cell borders. At this stage, significant pools of N\u2010 and E\u2010cadherins were Triton\u2010soluble, suggesting that fractions of these molecules were not localized to stable junctional complexes associated with the actin cytoskeleton. With cytodifferentiation, cadherins became progressively Triton\u2010insoluble, and this correlated with their organization at cell\u2013cell interfaces. In the cytodifferentiated SMG, N\u2010cadherin was absent, whereas E\u2010cadherin remained at cell\u2013cell interfaces. Early in morphogenesis, \u03b2\u2010catenin was also primarily Triton\u2010soluble, and its association with the actin cytoskeleton and localization to the adherens junctions increased with cytodifferentiation. Greater recruitment of cadherins and \u03b2\u2010catenin to cell\u2013cell borders was paralleled by changes in membrane association of two Rho GTPases, Cdc42 and RhoA. N\u2010cadherin was detected only at early stages of postnatal development, whereas E\u2010cadherin and \u03b2\u2010catenin became progressively Triton\u2010insoluble during differentiation. Our results indicate that N\u2010cadherin functions transiently in SMG development. On the other hand, E\u2010cadherin and \u03b2\u2010catenin appear to play different roles during tissue organization and cytodifferentiation. In early morphogenesis, E\u2010cadherin and \u03b2\u2010catenin are likely to participate in SMG remodeling, whereas during cytodifferentiation, they form stable cell\u2013cell contacts, and may collaborate with Rho GTPases in the establishment and maintenance of salivary cell polarity. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10111","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T07:34:39Z","timestamp":1033889679000},"page":"321-333","source":"Crossref","is-referenced-by-count":31,"title":["Regulation of cadherin junctions during mouse submandibular gland development"],"prefix":"10.1002","volume":"224","author":[{"given":"A. 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