{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:03:31Z","timestamp":1790705011947,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,6,13]],"date-time":"2002-06-13T00:00:00Z","timestamp":1023926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Titf2\/foxe1<\/jats:italic> is a forkhead domain\u2010containing gene expressed in the foregut, in the thyroid, and in the cranial ectoderm of the developing mouse. <jats:italic>Titf2<\/jats:italic> null mice exhibit cleft palate and either a sublingual or completely absent thyroid gland. In humans, mutations of the gene encoding for thyroid transcription factor\u20102 (TTF\u20102) result in the Bamforth syndrome, characterized by thyroid agenesis, cleft palate, spiky hair, and choanal atresia. Here, we report a detailed expression pattern of TTF\u20102 protein during mouse embryogenesis and show its presence in structures where it has not been described yet. At embryonic day (E) 10.5, TTF\u20102 is expressed in Rathke's pouch, in thyroid, and in the epithelium of the pharyngeal wall and arches, whereas it is absent in the epithelium of the pharyngeal pouches. According to this expression, at E13.5, TTF\u20102 is present in endoderm derivatives, such as tongue, palate, epiglottis, pharynx, and oesophagus. Later in embryogenesis, we detect TTF\u20102 in the choanae and whiskers. This pattern of expression helps to define the complex phenotype displayed by human patients. Finally, we show that TTF\u20102 is a phosphorylated protein. These results help to characterize the domains of TTF\u20102 expression, from early embryogenesis throughout organogenesis, providing more detail on the potential role of TTF\u20102 in the development of endoderm and ectoderm derived structures. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10118","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T07:53:08Z","timestamp":1033890788000},"page":"450-456","source":"Crossref","is-referenced-by-count":81,"title":["Distribution of the <i>titf2\/foxe1<\/i> gene product is consistent with an important role in the development of foregut endoderm, palate, and hair"],"prefix":"10.1002","volume":"224","author":[{"given":"Nina","family":"Dathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosanna","family":"Parlato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annamaria","family":"Rosica","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"De Felice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Di 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for normal forebrain, liver, and thyroid formation","volume":"127","author":"Martinez Barbera JP","year":"2000","journal-title":"Development"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1242\/dev.119.3.567","article-title":"Postimplantation expression patterns indicate a role for the mouse forkhead\/HNF\u20103 \u03b1, \u03b2, and \u03b3 genes in determination of the definitive endoderm, chordamesoderm, and neuroectoderm","volume":"119","author":"Monaghan AP","year":"1993","journal-title":"Development"},{"key":"e_1_2_5_17_1","first-page":"2118","article-title":"TTF\u20102, a new forkhead protein, shows a temporal expression in the developing thyroid which is consistent with a role in controlling the onset of differentiation \u2010 Corrigendum","volume":"20","author":"Parlato R","year":"2001","journal-title":"EMBO 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