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In an attempt to delineate the regulatory mechanism of the \u03b12B receptor subtype (encoded by subtype gene <jats:italic>Adra2b<\/jats:italic>) expression in the central nervous system (CNS), we have established transgenic (Tg) mice lines in which the transgene (NN\u2010<jats:italic>lacZ<\/jats:italic>) was composed of the promoter region of <jats:italic>Adra2b<\/jats:italic> (<jats:italic>Nco<\/jats:italic>I fragment, 4.7 kb immediately upstream from receptor coding region) and a reporter gene <jats:italic>lacZ<\/jats:italic> (encoding \u03b2\u2010galactosidase). The selective expression of \u03b12B in brain as indexed by \u03b2\u2010galactosidase, under the direction of this promoter region, may be traced in situ by using X\u2010gal staining. The expression pattern of <jats:italic>Adra2b<\/jats:italic>\u2010NN\u2010<jats:italic>lacZ<\/jats:italic> in CNS of Tg mice during development was examined. The temporal course of examination was from gestation day 9.5 (E9.5) to postnatal day 28 (P28). Significant X\u2010gal staining was detected in the dorsal root ganglion and cranial nerves V and VII at E12.5. By E18.5, expression was noted in the cerebral cortex, anterior olfactory nucleus, hypothalamus, brainstem, and cerebellar Purkinje cells, among others, and persisted through postnatal development. <jats:italic>Adra2b<\/jats:italic>\u2010NN\u2013directed reporter expression was detected in the hippocampal dentate gyrus first at P4. The temporal course of expression up to P28 in this area is in accordance with the developmental profiles of granule neurons of dentate gyrus. From P7 on, transgene expression was detected in additional brain areas, including the septum and thalamus. The expression correlates well with the noradrenergic innervations as evidenced by colocalization by using tyrosine hydroxylase or dopamine\u2010\u03b2\u2010hydroxylase immunocytochemistry. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10141","type":"journal-article","created":{"date-parts":[[2002,10,8]],"date-time":"2002-10-08T22:46:26Z","timestamp":1034117186000},"page":"142-152","source":"Crossref","is-referenced-by-count":22,"title":["Regulated expression of \u03b12B adrenoceptor during development"],"prefix":"10.1002","volume":"225","author":[{"given":"Guey\u2010Shin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan\u2010Chi","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinn\u2010Chih","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice 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