{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T23:01:14Z","timestamp":1789599674134,"version":"build-2803163510"},"reference-count":79,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,3,28]],"date-time":"2003-03-28T00:00:00Z","timestamp":1048809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Tg737<\/jats:italic> mutant mice exhibit pathologic conditions in numerous tissues along with skeletal patterning defects. Herein, we characterize the skeletal pathologic conditions and confirm a role for <jats:italic>Tg737<\/jats:italic> in skeletal patterning through transgenic rescue. Analyses were conducted in both the hypomorphic <jats:italic>Tg737<\/jats:italic><jats:sup><jats:italic>orpk<\/jats:italic><\/jats:sup> allele that results in duplication of digit one and in the null <jats:italic>Tg737<\/jats:italic><jats:sup><jats:italic>\u03942\u20103\u03b2Gal<\/jats:italic><\/jats:sup> allele that is an embryonic lethal mutation exhibiting eight digits per limb. In early limb buds, <jats:italic>Tg737<\/jats:italic> expression is detected throughout the mesenchyme becoming concentrated in precartilage condensations at later stages. In situ analyses indicate that the <jats:italic>Tg737<\/jats:italic><jats:sup><jats:italic>orpk<\/jats:italic><\/jats:sup> mutant limb defects are not associated with changes in expression of <jats:italic>Shh<\/jats:italic>, <jats:italic>Ihh<\/jats:italic>, <jats:italic>HoxD11\u201313<\/jats:italic>, <jats:italic>Patched<\/jats:italic>, <jats:italic>BMPs<\/jats:italic>, or <jats:italic>Glis<\/jats:italic>. Likewise, in <jats:italic>Tg737<\/jats:italic><jats:sup><jats:italic>\u03942\u20103\u03b2Gal<\/jats:italic><\/jats:sup> mutant embryos, there was no change in <jats:italic>Shh<\/jats:italic> expression. However, in both alleles, <jats:italic>Fgf4<\/jats:italic> was ectopically expressed on the anterior apical ectodermal ridge. Collectively, the data argue for a dosage effect of <jats:italic>Tg737<\/jats:italic> on the limb phenotypes and that the polydactyly is independent of <jats:italic>Shh<\/jats:italic> misexpression. Developmental Dynamics 227:78\u201390, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10289","type":"journal-article","created":{"date-parts":[[2003,4,14]],"date-time":"2003-04-14T06:03:01Z","timestamp":1050300181000},"page":"78-90","source":"Crossref","is-referenced-by-count":115,"title":["Loss of the <i>Tg737<\/i> protein results in skeletal patterning defects"],"prefix":"10.1002","volume":"227","author":[{"given":"Qihong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noel S.","family":"Murcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura R.","family":"Chittenden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William G.","family":"Richards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward 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