{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:04:00Z","timestamp":1767841440960,"version":"3.49.0"},"reference-count":9,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,11,12]],"date-time":"2003-11-12T00:00:00Z","timestamp":1068595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Ehox<\/jats:italic> is an X\u2010linked paired like homeobox gene identified from a differentiating embryonic stem (ES) cell cDNA library and is expressed at low levels in the preimplantation blastocyst and in ES cells in vitro. In embryos at 6.5 days post coitum (dpc), <jats:italic>Ehox<\/jats:italic> expression was restricted to the extraembryonic ectoderm which correlates with high\u2010level expression in cultures of trophoblast stem cells. Extraembryonic expression becomes further restricted to the chorion and by 15.5 dpc <jats:italic>Ehox<\/jats:italic> is expressed in chorionic trophoblast of the labyrinth and spongiotrophoblast layers of the placenta. <jats:italic>Ehox<\/jats:italic> expression in the embryo proper first appears at 8.5 dpc in the anterior foregut endoderm and by 9.5 dpc is visible in pharyngeal pouches 2\u20134. By 10.5 dpc, <jats:italic>Ehox<\/jats:italic> expression becomes restricted to the ventral end of pouches 2 and 3. The data presented here is the first description of <jats:italic>Ehox<\/jats:italic> expression during embryogenesis and suggests a dual role for <jats:italic>Ehox<\/jats:italic>: (1) in trophoblast stem cells and compartments of the developing placenta, and (2) during development of the pharyngeal pouches, possibly delineating the area to become thymus. Development Dynamics 228:740\u2013744, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.10413","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T09:21:41Z","timestamp":1070356901000},"page":"740-744","source":"Crossref","is-referenced-by-count":15,"title":["Expression of a novel homeobox gene <i>Ehox<\/i> in trophoblast stem cells and pharyngeal pouch endoderm"],"prefix":"10.1002","volume":"228","author":[{"given":"Melany","family":"Jackson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet W.","family":"Baird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Nichols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Wilkie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Ansell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerry","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lesley M.","family":"Forrester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,11,12]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008354200"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90248-J"},{"key":"e_1_2_4_4_1","first-page":"156","article-title":"Single step method of isolation of RNA by acid\u2010guanidinium\u2010phenol\u2010chloroform extraction","volume":"161","author":"Chomcznski P","year":"1987","journal-title":"Annal Biochem"},{"key":"e_1_2_4_5_1","unstructured":"HoganB BeddingtonR ConstantiniF LacyE.1994.Manipulating the mouse embryo: a laboratory manual. 2nd ed."},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203459200"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.21.4891"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81769-9"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2072"},{"key":"e_1_2_4_10_1","volume-title":"In situ hybridisation","author":"Wilkinson DG","year":"1992"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.10413","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.10413","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T16:02:28Z","timestamp":1760371348000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.10413"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11,12]]},"references-count":9,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1002\/dvdy.10413"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.10413","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11,12]]}}}