{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:06:13Z","timestamp":1779235573706,"version":"3.51.4"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,2,26]],"date-time":"2001-02-26T00:00:00Z","timestamp":983145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mammalian submandibular gland (SMG) development leads to the establishment of highly organized secretory acinar and nonsecretory ductal epithelial cells. The ability of maturing salivary epithelial cells to attain their differentiated state has been shown to depend, in part, on interactions between extracellular matrix (ECM) proteins and their integrin receptors. In a search for key regulators of salivary cell lineage, we have studied \u03b13\u03b21 integrin, a receptor for the basement membrane protein laminin, by characterizing embryonic day 18 (E18) SMGs isolated from mice carrying a targeted mutation in the \u03b13 integrin gene. Transmission electron microscopy studies showed that the mutant SMGs exhibited an aberrant differentiation phenotype with defects in the apical\u2010basal polarity axis and in the basement membrane. Based on immunohistochemistry and Western blot analyses, the \u03b13\u03b21\u2010deficient SMGs had altered expression and\/or localization of several ECM and adhesive molecules, including laminin \u03b21, fibronectin, \u03b15 integrin, and E\u2010cadherin. These changes correlated with alterations in the activation state of Ras\u2010extracellular signal\u2010regulated kinase (ERK), as well as the expression and\/or localization of Cdc42 and RhoA, two Rho GTPases that regulate the organization of the actin cytoskeleton. We conclude that \u03b13\u03b21 is required for normal salivary cell differentiation and that its absence affects multiple components of adhesive complexes and their associated signalling pathways. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.1114","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T16:21:39Z","timestamp":1030292499000},"page":"337-349","source":"Crossref","is-referenced-by-count":50,"title":["Loss of \u03b13\u03b21 integrin function results in an altered differentiation program in the mouse submandibular gland"],"prefix":"10.1002","volume":"220","author":[{"given":"A. 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