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However, its role in fin regeneration remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>mmp13a<\/jats:italic> expression is strongly upregulated during blastema formation and persists in the distal blastema. <jats:italic>mmp13a<\/jats:italic> knockdown via morpholino electroporation impairs regenerative outgrowth by decreasing cell proliferation, which correlates with a downregulation of <jats:italic>fgf10a<\/jats:italic> and <jats:italic>sall4<\/jats:italic> expression in the blastema. Laminin distribution in the basement membrane is also affected in <jats:italic>mmp13a<\/jats:italic> MO\u2010injected rays. Another impact of <jats:italic>mmp13a<\/jats:italic> knockdown is observed in the skeletal elements of the fin rays. Expression of two main components of actinotrichia, Collagen II and Actinodin 1 is highly reduced in <jats:italic>mmp13a<\/jats:italic> MO\u2010injected rays leading to highly disorganized actinotrichia pattern. Inhibition of <jats:italic>mmp13a<\/jats:italic> strongly affects bone formation as shown by a reduction of Zns5 and <jats:italic>sp7<\/jats:italic> expression and of bone matrix mineralization in rays. These defects are accompanied by a significant increase in apoptosis in <jats:italic>mmp13a<\/jats:italic> MO\u2010injected fin regenerates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Defects of expression of this multifunctional proteinase drastically affects osteoblast differentiation, bone and actinotrichia formation as well as Laminin distribution in the basement membrane of the fin regenerate, suggesting the important role of Mmp13 during the regenerative process.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.112","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T11:14:30Z","timestamp":1567682070000},"page":"187-198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Inhibition of <i>mmp13a<\/i> during zebrafish fin regeneration disrupts fin growth, osteoblasts differentiation, and Laminin 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