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Although staining protocols using phosphotungstic acid (PTA) have been published with beautiful visualizations of soft tissue structures, these protocols are often aimed at highly specific research questions and are applicable to a limited set of model organisms, specimen ages, or tissue types. We provide detailed protocols for micro\u2010level visualization of soft tissue structures in mice at several embryonic and early postnatal ages using PTA\u2010enhanced microCT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our protocols produce microCT scans that enable visualization and quantitative analyses of whole organisms, individual tissues, and organ systems while preserving 3D morphology and relationships with surrounding structures, with minimal soft tissue shrinkage. Of particular note, both internal and external features of the murine heart, lungs, and liver, as well as embryonic cartilage, are captured at high resolution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These protocols have broad applicability to mouse models for a variety of diseases and conditions. Minor experimentation in the staining duration can expand this protocol to additional age groups, permitting ontogenetic studies of internal organs and soft tissue structures within their 3D in situ position.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.136","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T04:12:45Z","timestamp":1574050365000},"page":"573-585","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Phosphotungstic acid\u2010enhanced microCT: Optimized protocols for embryonic and early postnatal mice"],"prefix":"10.1002","volume":"249","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9537-5750","authenticated-orcid":false,"given":"Kate M.","family":"Lesciotto","sequence":"first","affiliation":[{"name":"Department of Anthropology Pennsylvania State University  University Park Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan 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