{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T01:12:06Z","timestamp":1784941926718,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,12,14]],"date-time":"2019-12-14T00:00:00Z","timestamp":1576281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["17\u201014886S"],"award-info":[{"award-number":["17\u201014886S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In mammals, odontogenesis is regulated by transient signaling centers known as enamel knots (EKs), which drive the dental epithelium shaping. However, the developmental mechanisms contributing to formation of complex tooth shape in reptiles are not fully understood. Here, we aim to elucidate whether signaling organizers similar to EKs appear during reptilian odontogenesis and how enamel ridges are formed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Morphological structures resembling the mammalian EK were found during reptile odontogenesis. Similar to mammalian primary EKs, they exhibit the presence of apoptotic cells and no proliferating cells. Moreover, expression of mammalian EK\u2010specific molecules (SHH, FGF4, and ST14) and GLI2\u2010negative cells were found in reptilian EK\u2010like areas. 3D analysis of the nucleus shape revealed distinct rearrangement of the cells associated with enamel groove formation. This process was associated with ultrastructural changes and lipid droplet accumulation in the cells directly above the forming ridge, accompanied by alteration of membranous molecule expression (Na\/K\u2010ATPase) and cytoskeletal rearrangement (F\u2010actin).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The final complex shape of reptilian teeth is orchestrated by a combination of changes in cell signaling, cell shape, and cell rearrangement. All these factors contribute to asymmetry in the inner enamel epithelium development, enamel deposition, ultimately leading to the formation of characteristic enamel ridges.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.138","type":"journal-article","created":{"date-parts":[[2019,11,24]],"date-time":"2019-11-24T23:39:30Z","timestamp":1574638770000},"page":"441-464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Developmental mechanisms driving complex tooth shape in reptiles"],"prefix":"10.1002","volume":"249","author":[{"given":"Marie","family":"Landova Sulcova","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics Czech Academy of Science  Brno Czech Republic"},{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oldrich","family":"Zahradnicek","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine Czech Academy of Science  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Dumkova","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Faculty of Medicine, Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Dosedelova","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics Czech Academy of Science  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Krivanek","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Faculty of Medicine, Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Hampl","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics Czech Academy of Science  Brno Czech Republic"},{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7435-9292","authenticated-orcid":false,"given":"Michaela","family":"Kavkova","sequence":"additional","affiliation":[{"name":"CEITEC\u2010Central European Institute of Technology University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2948-5198","authenticated-orcid":false,"given":"Tomas","family":"Zikmund","sequence":"additional","affiliation":[{"name":"CEITEC\u2010Central European Institute of Technology University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4982-4524","authenticated-orcid":false,"given":"Martina","family":"Gregorovicova","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Medical Faculty Charles University  Prague Czech Republic"},{"name":"Institute of Physiology Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6828-3671","authenticated-orcid":false,"given":"David","family":"Sedmera","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Medical Faculty Charles University  Prague Czech Republic"},{"name":"Institute of Physiology Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7397-125X","authenticated-orcid":false,"given":"Jozef","family":"Kaiser","sequence":"additional","affiliation":[{"name":"CEITEC\u2010Central European Institute of Technology University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8871-6094","authenticated-orcid":false,"given":"Abigail S.","family":"Tucker","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine Czech Academy of Science  Prague Czech Republic"},{"name":"Centre for Craniofacial and Regenerative Biology Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0262-6774","authenticated-orcid":false,"given":"Marcela","family":"Buchtova","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics Czech Academy of Science  Brno Czech Republic"},{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00074"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1956.tb01551.x"},{"key":"e_1_2_7_4_1","first-page":"261","article-title":"Aspects of cell proliferation kinetics of the inner dental epithelium during mouse molar and incisor morphogenesis: a reappraisal of the role of the enamel knot area","volume":"43","author":"Coin R","year":"1999","journal-title":"Int J Dev Biol"},{"key":"e_1_2_7_5_1","first-page":"463","article-title":"Evidence for the role of the enamel knot as a control center in mammalian tooth cusp formation \u2010 nondividing cells express growth\u2010stimulating Fgf\u20104 gene","volume":"38","author":"Jernvall J","year":"1994","journal-title":"Int J Dev Biol"},{"key":"e_1_2_7_6_1","first-page":"1017","article-title":"Mouse molar morphogenesis revisited by three\u2010dimensional reconstruction .2. Spatial distribution of mitoses and apoptosis in cap to bell staged first and second upper molar teeth","volume":"40","author":"Lesot H","year":"1996","journal-title":"Int J Dev Biol"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(95)00459-9"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199712)210:4<383::AID-AJA3>3.0.CO;2-C"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.21.4325"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.10.3035"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.22.4775"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00095-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209509013701"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(97)00115-9"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.130187"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-015-0557-0"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2016.01.038"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1986.tb03657.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1987.tb05984.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.10.019"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21438"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2012.01531.x"},{"key":"e_1_2_7_23_1","first-page":"121","article-title":"Molecular characterization of dental development in a toothed archosaur, the American alligatorAlligator mississippiensis (vol 15, pg 393, 2013)","volume":"16","author":"Weeks O","year":"2014","journal-title":"Evol Dev"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.09.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.03.004"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500506"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540305100805"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.15.2803"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029181"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290299"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.2.161"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-007-9132-3"},{"key":"e_1_2_7_33_1","first-page":"12","article-title":"Fine tuning of Rac1 and RhoA alters cuspal shapes by remolding the cellular geometry","volume":"6","author":"Li LW","year":"2016","journal-title":"Sci Rep"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00400.2003"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205502"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddiscovery.2017.75"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14152"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.138","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvdy.138","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T18:43:48Z","timestamp":1760381028000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,14]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1002\/dvdy.138"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.138","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,14]]},"assertion":[{"value":"2019-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}