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Intraflagellar transport protein 20 (IFT20) is a master regulator for the control of spermatogenesis and male fertility in mice. However, the mechanism of how IFT20 regulates spermatogenesis is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Spermatogenesis associated 1 (SPATA1) was identified to be a major potential binding partner of IFT20 by a yeast two\u2010hybrid screening. The interaction between SPATA1 and IFT20 was examined by direct yeast two\u2010hybrid, co\u2010localization, and co\u2010immunoprecipitation assays. SPATA1 is highly abundant in the mouse testis, and is also expressed in the heart and kidney. During the first wave of spermatogenesis, SPATA1 is detectable at postnatal day 24 and its expression is increased at day 30 and 35. Immunofluorescence staining of mouse testis sections and epididymal sperm demonstrated that SPATA1 is localized mainly in the acrosome of developing spermatids but not in epididymal sperm. IFT20 is also present in the acrosome area of round spermatids. In conditional <jats:italic>Ift20<\/jats:italic> knockout mice, testicular expression level and acrosomal localization of SPATA1 are not changed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SPATA1 is an IFT20 binding protein and may provide a docking site for IFT20 complex binding to the acrosome area.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.141","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T10:49:44Z","timestamp":1575888584000},"page":"543-555","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mouse spermatogenesis\u2010associated protein 1 (SPATA1), an IFT20 binding partner, is an acrosomal protein"],"prefix":"10.1002","volume":"249","author":[{"given":"Ling","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Occupational and Environmental Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control School of Public Health, Wuhan University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingkai","family":"Zhen","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control School of Public Health, Wuhan University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, Hubei Province Key Laboratory of Occupational Hazard Identification and Control School of Public Health, Wuhan University of Science and Technology  Wuhan China"},{"name":"Department of Physiology Wayne State University  Detroit 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