{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:28:28Z","timestamp":1760401708143,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,10]],"date-time":"2020-03-10T00:00:00Z","timestamp":1583798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Colonic atresias in the <jats:italic>Fibroblast growth factor receptor 2IIIb (Fgfr2IIIb)<\/jats:italic> mouse model have been attributed to increased epithelial apoptosis and decreased epithelial proliferation at embryonic day (E) 10.5. We therefore hypothesized that these processes would colocalize to the distal colon where atresias occur (atretic precursor) and would be excluded or minimized from the proximal colon and small intestine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed a global increase in intestinal epithelial apoptosis in <jats:italic>Fgfr2IIIb<\/jats:italic><jats:sup>\n<jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> intestines from E9.5 to E10.5 that did not colocalize to the atretic precursor. Additionally, epithelial proliferations rates in <jats:italic>Fgfr2IIIb<\/jats:italic><jats:sup>\n<jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> intestines were statistically indistinguishable to that of controls at E10.5 and E11.5. At E11.5 distal colonic epithelial cells in mutants failed to assume the expected pseudostratified columnar architecture and the continuity of the adjacent basal lamina was disrupted. Individual E\u2010cadherin\u2010positive cells were observed in the colonic mesenchyme.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our observations suggest that alterations in proliferation and apoptosis alone are insufficient to account for intestinal atresias and that these defects may arise from both a failure of distal colonic epithelial cells to develop normally and local disruptions in basal lamina architecture.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.164","type":"journal-article","created":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T07:21:25Z","timestamp":1582701685000},"page":"741-753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lack of discreet colocalization of epithelial apoptosis to the atretic precursor in the colon of the <i>Fibroblast growth factor receptor 2IIIb<\/i> mouse and staining consistent with cellular movement suggest a revised model of atresia formation"],"prefix":"10.1002","volume":"249","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1902-7890","authenticated-orcid":false,"given":"Anna","family":"Kowalkowski","sequence":"first","affiliation":[{"name":"Surgery Department University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof M.","family":"Zaremba","sequence":"additional","affiliation":[{"name":"Surgery Department University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew P.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Surgery Department University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivia R.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Surgery Department University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne E.","family":"Turco","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"Nichol","sequence":"additional","affiliation":[{"name":"Surgery Department University of Wisconsin  Madison Wisconsin USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,10]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"ICBDSR. 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