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To elucidate temporospatial aspects of <jats:italic>Relb<\/jats:italic> expression, we generated a BAC transgenic knock\u2010in mouse expressing the fluorescent protein Katushka and the enzyme Cre recombinase under control of the murine <jats:italic>Relb<\/jats:italic> promoter (<jats:italic>Relb<\/jats:italic><jats:sup><jats:italic>Cre\u2010Kat<\/jats:italic><\/jats:sup> mice).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Co\u2010expression of <jats:italic>Katushka<\/jats:italic> and <jats:italic>Relb<\/jats:italic> in fibroblast cultures and tissues of transgenic mice revealed highly specific reporter functions of the transgene. Crossing <jats:italic>Relb<\/jats:italic><jats:sup><jats:italic>Cre\u2010Kat<\/jats:italic><\/jats:sup> mice with <jats:italic>ROSA26R<\/jats:italic> reporter mice that allow for Cre\u2010mediated consecutive \u03b2\u2010galactosidase or YFP synthesis identified various <jats:italic>Relb<\/jats:italic> expression domains in perinatal and mature mice. Besides thymus and spleen, highly specific expression patterns were found in different neuronal domains, as well as in other nonimmune organs including skin, skeletal structures and kidney. De novo <jats:italic>Relb<\/jats:italic> expression in the mature brain was confirmed in conditional knockout mice with neuro\u2010ectodermal <jats:italic>Relb<\/jats:italic> deletion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate the usability of <jats:italic>Relb<\/jats:italic><jats:sup><jats:italic>Cre\u2010Kat<\/jats:italic><\/jats:sup> reporter mice for the detection of de novo and temporarily restricted <jats:italic>Relb<\/jats:italic> expression including cell and lineage tracing of <jats:italic>Relb<\/jats:italic> expressing cells. <jats:italic>Relb<\/jats:italic> expression during mouse embryogenesis and at adulthood suggests, beyond immunity, important functions of this transcription factor in neurodevelopment and CNS function.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.170","type":"journal-article","created":{"date-parts":[[2020,3,7]],"date-time":"2020-03-07T11:49:42Z","timestamp":1583581782000},"page":"983-997","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of undescribed <i>Relb<\/i> expression domains in the murine brain by new <i>Relb:cre\u2010katushka<\/i> reporter mice"],"prefix":"10.1002","volume":"249","author":[{"given":"Christian","family":"Engelmann","sequence":"first","affiliation":[{"name":"Leibniz Institute on Aging \u2013 Fritz Lipmann Institute (FLI)  Jena Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Riemann","sequence":"additional","affiliation":[{"name":"Leibniz Institute on Aging \u2013 Fritz Lipmann Institute (FLI)  Jena 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