{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T05:30:20Z","timestamp":1773207020593,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T00:00:00Z","timestamp":1582588800000},"content-version":"am","delay-in-days":343,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FT110100496"],"award-info":[{"award-number":["FT110100496"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1083811"],"award-info":[{"award-number":["1083811"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1106800"],"award-info":[{"award-number":["1106800"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>During heart morphogenesis, the cardiac chambers undergo ballooning: a process involving regionalized elongation of cardiomyocytes. Cardiomyocyte shape changes require reorganization of the actin cytoskeleton; however, the genetic regulation of this process is not well understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>From a forward genetic screen, we identified the zebrafish <jats:italic>uq<\/jats:italic><jats:sup>\n<jats:italic>23ks<\/jats:italic><\/jats:sup> mutant which manifests chamber ballooning defects. Whole\u2010genome sequencing\u2010mapping identified a truncating mutation in the gene, <jats:italic>myo5b. myo5b<\/jats:italic> encodes an atypical myosin required for endosome recycling and, consistent with this, increased vesicles were observed in <jats:italic>myo5b<\/jats:italic> mutant cardiomyocytes. Expression of RFP\u2010Rab11a (a recycling endosome marker) confirmed increased recycling endosomes in cardiomyocytes of <jats:italic>myo5b<\/jats:italic> mutants. To investigate potential cargo of MyoVb\u2010associated vesicles, we examined the adherens junction protein, N\u2010cadherin. N\u2010cadherin appeared mispatterned at cell junctions, and an increase in the number of intracellular particles was also apparent. Co\u2010localization with RFP\u2010Rab11a confirmed increased N\u2010cadherin\u2010positive recycling endosomes, demonstrating N\u2010cadherin trafficking is perturbed in <jats:italic>myo5b<\/jats:italic> mutants. Finally, phalloidin staining showed disorganized F\u2010actin in <jats:italic>myo5b<\/jats:italic> cardiomyocytes, suggesting the cytoskeleton fails to remodel, obstructing chamber ballooning.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MyoVb is required for cardiomyocyte endosomal recycling and appropriate N\u2010cadherin localization during the onset of chamber ballooning. Cardiomyocytes lacking MyoVb are unable to reorganize their actin cytoskeleton, resulting in failed chamber ballooning. Developmental Dynamics 248:284\u2013295, 2019. \u00a9 2019 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.19","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T00:30:53Z","timestamp":1551141053000},"page":"284-295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Myosin Vb is required for correct trafficking of N\u2010cadherin and cardiac chamber ballooning"],"prefix":"10.1002","volume":"248","author":[{"given":"Daniela R.","family":"Grassini","sequence":"first","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"da Silva","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Hall","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J.","family":"Baillie","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cas","family":"Simons","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"},{"name":"Murdoch Children's Research Institute  Parkville Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert G.","family":"Parton","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin M.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8283-9760","authenticated-orcid":false,"given":"Kelly A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  Brisbane Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050053"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-6-23"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3456"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.020974"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12312"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22758"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002861"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.12.017"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.050526"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23386"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00462.2010"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a027979"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124214"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcg.0000225632.07039.b6"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.160739"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000145855"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00305115"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.03.068"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.263210.115"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000156274.72390.2c"},{"key":"e_1_2_6_22_1","volume-title":"Heart development: interactions involved in cardiac morphogenesis","author":"Manasek FJ","year":"1976"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(72)90077-2"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGENETICS.116.001605"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.225"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.003830"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3754"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.048439"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.10.003"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.8443"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1516672112"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302711"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1756"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.11.015"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.065375"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004614"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000239407.45137.97"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.052045lt"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.164194"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.06.023"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2900"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dvdy.19","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/dvdy.19","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:13:25Z","timestamp":1760343205000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,19]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/dvdy.19"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.19","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,19]]},"assertion":[{"value":"2018-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}