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We find that <jats:italic>pouC<\/jats:italic> is a multi\u2010functional transcriptional regulator containing separable activation, repression, protein\u2013protein interaction, and DNA binding domains. Using zebrafish heart development as a model system, we demonstrate that <jats:italic>pouC<\/jats:italic> knockdown impairs cardiac morphogenesis and affects cardiovascular function. We also find that levels of <jats:italic>pouC<\/jats:italic> expression must be fine\u2010tuned to enable proper heart formation. At the cellular level, we demonstrate that <jats:italic>pouC<\/jats:italic> knockdown disrupts atrioventricular canal (AVC) cardiomyocyte maintenance, although chamber myocyte specification remains intact. Mechanistically, we show that <jats:italic>pouC<\/jats:italic> binds a <jats:italic>bmp4<\/jats:italic> intronic regulatory element to mediate transcriptional activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Taken together, our study establishes <jats:italic>pouC<\/jats:italic> as a novel transcriptional input into the regulatory hierarchy that drives AVC morphogenesis in zebrafish. We anticipate that these findings will inform future efforts to explore functional conservation in mammals and potential association with atrioventricular septal defects in humans. <jats:italic>Developmental Dynamics<\/jats:italic> 248:173\u2010188, 2019. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.2","type":"journal-article","created":{"date-parts":[[2018,11,16]],"date-time":"2018-11-16T09:46:47Z","timestamp":1542361607000},"page":"173-188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>pouC<\/i> Regulates Expression of <i>bmp4<\/i> During Atrioventricular Canal Formation in Zebrafish"],"prefix":"10.1002","volume":"248","author":[{"given":"Minoti","family":"Bhakta","sequence":"first","affiliation":[{"name":"Department of Internal Medicine \u2013 Cardiology UT Southwestern Medical Center  Dallas Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahesh 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