{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T06:48:24Z","timestamp":1760424504676,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,1,28]],"date-time":"2004-01-28T00:00:00Z","timestamp":1075248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ciliary epithelium of the ciliary body is derived from the anterior rim of the developing optic cup. Several recent studies have found that developmental abnormalities in this tissue can underlie congenital glaucoma. However, there is little known about the development of the ciliary epithelium. To better understand the developmental events responsible for the specification of this domain of the optic cup, we used a subtractive library, differential screening approach along with the construction of cDNA arrays to identify genes expressed in the ciliary epithelium of the chicken. We identified many genes specifically expressed in the ciliary epithelium, including a number that had been described previously as enriched in the ciliary epithelium of other species. By analyzing the expression of these genes during eye development, we were able to correlate the onset of ciliary epithelial gene expression with a reduction in mitotic activity in this region. We propose that the mechanisms that regulate the expression of ciliary epithelial genes are linked to the reduction in proliferation that results in the epithelial monolayer in this region. Developmental Dynamics 229:529\u2013540, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20000","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T15:29:18Z","timestamp":1077809358000},"page":"529-540","source":"Crossref","is-referenced-by-count":26,"title":["Identification of ciliary epithelial\u2010specific genes using subtractive libraries and cDNA arrays in the avian eye"],"prefix":"10.1002","volume":"229","author":[{"given":"Ryo","family":"Kubota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"McGuire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blair","family":"Dierks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas A.","family":"Reh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90153-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.5978"},{"key":"e_1_2_6_4_1","first-page":"123","article-title":"Development of the ciliary body: a brief review","volume":"105","author":"Beebe DC","year":"1986","journal-title":"Trans Ophthalmol Soc UK"},{"key":"e_1_2_6_5_1","first-page":"10","article-title":"A simplified in situ hybridization protocol using non\u2010radioactively labeled probes to detect abundant and rare mRNAs on tissue sections","volume":"1","author":"Braissant O","year":"1998","journal-title":"Biochemica"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-2-18"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.2002.1187"},{"key":"e_1_2_6_8_1","first-page":"470","article-title":"Differential localization of collagen type IX isoform messenger RNAs during early ocular development","volume":"35","author":"Dhawan RR","year":"1994","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"6025","DOI":"10.1073\/pnas.93.12.6025","article-title":"Suppression subtractive hybridization: a method for generating differentially regulated or tissue\u2010specific cDNA probes and libraries","volume":"12","author":"Diatchenko L","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10245"},{"key":"e_1_2_6_11_1","first-page":"315","article-title":"Bioinformatics and reanalysis of subtracted expressed sequence tags from the human ciliary body: identification of novel biological functions","volume":"8","author":"Escribano J","year":"2002","journal-title":"Mol Vis"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00178-7"},{"key":"e_1_2_6_13_1","first-page":"1193","article-title":"Expression and localization of angiogenic inhibitory factor, chondromodulin\u2010I, in adult rat eye","volume":"42","author":"Funaki H","year":"2001","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.51.32419"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00244"},{"key":"e_1_2_6_16_1","first-page":"1271","article-title":"Extracellular matrices of the developing chick retina and cornea. Localization of mRNAs for collagen types II and IX by in situ hybridization","volume":"31","author":"Linsenmayer TF","year":"1990","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(02)00115-X"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650100221"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200001)26:1<15::AID-GENE5>3.0.CO;2-V"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-0365"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.7.1021"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.081062398"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1122"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0140"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030410"},{"key":"e_1_2_6_26_1","first-page":"205","article-title":"Expressed sequence tag analysis of human RPE\/choroid for the NEIBank Project: over 6000 non\u2010redundant transcripts, novel genes and splice variants","volume":"8","author":"Wistow G","year":"2002","journal-title":"Mol Vis"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.19.4435"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.20000","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.20000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T19:16:45Z","timestamp":1760383005000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.20000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,28]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["10.1002\/dvdy.20000"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.20000","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"type":"print","value":"1058-8388"},{"type":"electronic","value":"1097-0177"}],"subject":[],"published":{"date-parts":[[2004,1,28]]}}}