{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:23:02Z","timestamp":1790972582988,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,6,3]],"date-time":"2004-06-03T00:00:00Z","timestamp":1086220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ghent University","award":["011V1298"],"award-info":[{"award-number":["011V1298"]}]},{"name":"\u2018Bijzonder Onderzoeksfonds\u2019 of Ghent University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>even<\/jats:italic>\u2010<jats:italic>skipped<\/jats:italic>\u2010related (<jats:italic>evx<\/jats:italic>) genes encode homeodomain\u2010containing transcription factors that are involved in a series of developmental processes such as posterior body patterning and neurodifferentiation. Although <jats:italic>evx1<\/jats:italic> and <jats:italic>evx2<\/jats:italic> were not reported to be expressed during mammalian tooth development, we present here evidence that <jats:italic>eve1<\/jats:italic>, the closest paralog of <jats:italic>evx1<\/jats:italic> in the actinopterygian lineage, is expressed during pharyngeal tooth formation in the zebrafish, <jats:italic>Danio rerio<\/jats:italic>. We have performed whole\u2010mount in situ hybridization on zebrafish embryos and larvae ranging from 24 to 192 hours postfertilization (hpf). A detailed analysis of serial sections through the pharyngeal region of whole\u2010mount hybridized and control specimens indicates that only dental epithelial cells express <jats:italic>eve1<\/jats:italic>. <jats:italic>eve1<\/jats:italic> transcription was activated at 48 hpf, in the placode of the first tooth (i.e., the initiation site of tooth 4V<jats:sup>1<\/jats:sup>), and maintained in the dental epithelium throughout morphogenesis. Then, by 72 hpf, <jats:italic>eve1<\/jats:italic> expression was restricted to the differentiating ameloblasts of the enamel organ during early differentiation stage, and this expression decreased as soon as matrix was deposited. In subsequent primary teeth (3 V<jats:sup>1<\/jats:sup> and 5 V<jats:sup>1<\/jats:sup>) as well as in their successors (replacement teeth 4V<jats:sup>2<\/jats:sup>, 3V<jats:sup>2<\/jats:sup>, and 5V<jats:sup>2<\/jats:sup>), <jats:italic>eve1<\/jats:italic> expression was restricted to the differentiating ameloblasts and, again, disappeared when matrix was deposited. Therefore, in the zebrafish, <jats:italic>eve1<\/jats:italic> expression in the pharyngeal region is correlated with two key steps of tooth development: initiation and morphogenesis of the first tooth, and ameloblast differentiation of all developing teeth. Developmental Dynamics 230:727\u2013733, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20080","type":"journal-article","created":{"date-parts":[[2004,6,3]],"date-time":"2004-06-03T17:00:49Z","timestamp":1086282049000},"page":"727-733","source":"Crossref","is-referenced-by-count":45,"title":["Cellular expression of <i>eve1<\/i> suggests its requirement for the differentiation of the ameloblasts and for the initiation and morphogenesis of the first tooth in the zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1002","volume":"230","author":[{"given":"Patrick","family":"Laurenti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Tha\u00ebron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Allizard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Huysseune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Yves","family":"Sire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5394.1711"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-142X.2003.03021.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020170204"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb08309.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00355726"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.10.3035"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(91)90482-T"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/333376a0"},{"key":"e_1_2_5_10_1","first-page":"143","article-title":"Developmental 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