{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:43:53Z","timestamp":1791251033727,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,8,9]],"date-time":"2004-08-09T00:00:00Z","timestamp":1092009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report that, during epiboly in zebrafish, three F\u2010actin\u2013based structures appear only after the blastoderm migrates past the embryonic equator. They are composed of two ring\u2010like F\u2010actin structures that form at the deep cell and enveloping layer margins of the blastoderm and a punctate actin band that develops in the external yolk syncytial layer. Treatment with cytochalasin B or the calcium chelator dibromo\u2010BAPTA results in the disruption of all three of these actin\u2010based structures, leading to the slowing or immediate arrest of epiboly, respectively, followed by a failure of yolk cell occlusion and the eventual lysis of the embryo through the vegetal pole region. We suggest, therefore, that these structures function in the occlusion of the vegetal portion of the yolk cell during the latter stages of epiboly. Possible roles for these new structures, their modulation by Ca<jats:sup>2+<\/jats:sup>, as well as the functions of other previously described F\u2010actin\u2013based structures observed throughout epiboly, are discussed. Developmental Dynamics 231:313\u2013323, 2004.\u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20144","type":"journal-article","created":{"date-parts":[[2004,8,10]],"date-time":"2004-08-10T03:15:06Z","timestamp":1092107706000},"page":"313-323","source":"Crossref","is-referenced-by-count":117,"title":["Organization and function of microfilaments during late epiboly in zebrafish embryos"],"prefix":"10.1002","volume":"231","author":[{"given":"Jackie C.","family":"Cheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew L.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,8,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80261-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402060310"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/26.1.193"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.1242\/jcs.111.12.1613","article-title":"Patterns of free calcium in zebrafish embryos","volume":"111","author":"Cr\u00e9ton R","year":"1998","journal-title":"J Cell Sci"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0294"},{"key":"e_1_2_6_7_1","first-page":"177","volume-title":"Motion analysis of living cells","author":"Cooper MS","year":"1998"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.1.157"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1016"},{"key":"e_1_2_6_10_1","first-page":"125","article-title":"Intercellular junctions, intramembranous particles and cytoskeletal elements of deep cells of the Fundulus gastrula","volume":"40","author":"Hogan JC","year":"1977","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00410-9"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402190213"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-46041-1_7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.123.1.47"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90099-6"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2003.710301.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002030302"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-169X.1998.t01-1-00007.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10087"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(02)00369-1"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050100203"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050041"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(89)90026-2"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.5.F111"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-46041-1_1"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(01)00297-6"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.9.2443"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.3.909"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401180204"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/24.3.673"},{"key":"e_1_2_6_31_1","doi-asserted-by":"crossref","first-page":"5375","DOI":"10.1242\/dev.00758","article-title":"Slb\/Wnt11 controls hypoblast cell migration and morphogenesis at the onset of zebrafish gastrulation","volume":"130","author":"Ulrich F","year":"2003","journal-title":"Development"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-10-0630"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.108.4.569"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199403002211"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8724"},{"key":"e_1_2_6_36_1","volume-title":"The zebrafish book. 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