{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:33:03Z","timestamp":1790389983771,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,9,16]],"date-time":"2004-09-16T00:00:00Z","timestamp":1095292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Embryonic development requires cell movements whose coordination is robust and reproducible. A dramatic example is the primary body axis of vertebrates: despite perturbation, cells in prospective axial tissue coordinate their movements to make an elongated body axis. The spatial cues coordinating these movements are not known. We show here that cells deprived of preexisting spatial cues by physical dissociation and reaggregation nonetheless organize themselves into an axis. Activin\u2010induced cells that are reaggregated into a flat disc initially round up into a ball before elongating perpendicular to the disc. Manipulations of the geometry of the disc and immunofluorescence micrography reveal that the edge of the disc provides a circumferential alignment zone. This finding indicates that physical boundaries provide alignment cues and that circumferential \u201choop stress\u201d drives the axial extrusion in a manner resembling late\u2010involuting mesoderm of <jats:italic>Xenopus<\/jats:italic> and archenteron elongation in other deuterostome species such as sea urchins. Thus, a population of cells finds its own midline based on the form of the population's boundaries using an edge\u2010aligning mechanism. This process provides a remarkably simple organizing principle that contributes to the reliability of embryonic development as a whole. Developmental Dynamics 231:576\u2013581, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20163","type":"journal-article","created":{"date-parts":[[2004,9,17]],"date-time":"2004-09-17T03:01:03Z","timestamp":1095390063000},"page":"576-581","source":"Crossref","is-referenced-by-count":37,"title":["Self\u2010organization of vertebrate mesoderm based on simple boundary conditions"],"prefix":"10.1002","volume":"231","author":[{"given":"Jeremy B.A.","family":"Green","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance A.","family":"Davidson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511626173"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.20.4547"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105.1.61"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.10.3311"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00848102"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.02.110186.001221"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-678-9:1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/347391a0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1242\/dev.108.1.173","article-title":"The biological effects of XTC\u2010MIF: quantitative comparison with Xenopus bFGF","volume":"108","author":"Green JBA","year":"1990","journal-title":"Development"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90550-V"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-46041-1_5"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90268-6"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90269-1"},{"key":"e_1_2_7_15_1","volume-title":"The early development of Xenopus laevis: an atlas of the histology","author":"Hausen P","year":"1991"},{"key":"e_1_2_7_16_1","first-page":"201","article-title":"The cellular basis of epiboly: an SEM study of deep\u2010cell rearrangement during gastrulation in Xenopus laevis","volume":"60","author":"Keller RE","year":"1980","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079478"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.1987.Supplement_8.21"},{"key":"e_1_2_7_19_1","first-page":"185","article-title":"The function and mechanism of convergent extension during gastrulation of Xenopus laevis","volume":"89","author":"Keller RE","year":"1985","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402510204"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2000.0626"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8420"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1242\/dev.124.4.895","article-title":"Microtubule disruption reveals that Spemann's organizer is subdivided into two domains by the vegetal alignment zone","volume":"124","author":"Lane MC","year":"1997","journal-title":"Development"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.18.3635"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.123.1.81"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/dev.129.1.1","article-title":"Morphogenetic pattern formation during ascidian notochord formation is regulative and highly robust","volume":"129","author":"Munro EM","year":"2002","journal-title":"Development"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02620"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/351409a0"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90388-8"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.101.2.339"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401280105"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1084-9521(02)00042-3"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402510212"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00197-1"},{"key":"e_1_2_7_35_1","first-page":"169","article-title":"Mesoderm induction in Xenopus. 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