{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:17:44Z","timestamp":1791274664263,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,21]],"date-time":"2004-10-21T00:00:00Z","timestamp":1098316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heparan sulfate (HS) interactions with secreted morphogens such as fibroblast growth factors, hedgehogs, and Wnts are essential for embryonic development. Formation of biologically relevant HS structures is a result of the coordinated action of various biosynthetic enzymes. HS 6\u2010<jats:italic>O<\/jats:italic>\u2010sulfotransferases (6OST) catalyze the transfer of sulfate groups to the 6\u2010<jats:italic>O<\/jats:italic> position of glucosamine residues in HS. Three 6OST isoforms have been described in the mouse; however, little is known about their role in generating specific HS protein\u2010binding sequences, expression pattern, and function in vivo. To gain insights into the distribution of these isoforms and their potential role in development, we mapped <jats:italic>6OST1\u20133<\/jats:italic> gene expression during mouse organogenesis. We report dynamic expression of these isoforms with striking differences in tissue distribution in many developing organs. We show that <jats:italic>6OST<\/jats:italic> transcripts are differentially expressed in several sites where heparin\u2010binding growth factors are critical for development. <jats:italic>6OST1<\/jats:italic> is predominantly transcribed in epithelial and neural\u2010derived tissues, whereas <jats:italic>6OST2<\/jats:italic> is more mesenchymal. <jats:italic>6OST3<\/jats:italic> appears at later stages and in a more restricted manner. The patterns reported here strongly suggest that the HS structures modified by these enzymes have different roles in growth factor\u2010induced developmental processes. Developmental Dynamics 231:782\u2013794, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20173","type":"journal-article","created":{"date-parts":[[2004,10,21]],"date-time":"2004-10-21T19:52:53Z","timestamp":1098388373000},"page":"782-794","source":"Crossref","is-referenced-by-count":55,"title":["Differential expression of heparan sulfate 6\u2010<i>O<\/i>\u2010sulfotransferase isoforms in the mouse embryo suggests distinctive roles during organogenesis"],"prefix":"10.1002","volume":"231","author":[{"given":"Jeff","family":"Sedita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Izvolsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wellington 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