{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T16:25:03Z","timestamp":1771259103657,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,12,21]],"date-time":"2004-12-21T00:00:00Z","timestamp":1103587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The temporal and spatial manipulation of gene expression is useful in analyzing the mechanisms of early embryogenesis. This report describes a modified strategy to achieve controlled gene expression by directed plasmid injection using the hsp70 promoter and heat treatment. Two control genes, enhanced green fluorescent protein (EGFP) and \u03b2\u2010catenin, were also expressed by this method. When embryos were injected with HsS1\/EGFP and subsequently heat\u2010treated, ectopic EGFP was expressed only in the injected area. No severe defects were attributable to the heat treatment alone. Western blotting confirmed that no EGFP induction occurred in the absence of heat treatment and that, in the presence of heat induction, EGFP expression was detected within 1 hr after treatment. These results suggest that heat\u2010mediated gene expression in the restricted area was regulated temporally. In addition, HsS1\/\u03b2\u2010catenin injection into the animal pole of 8\u2010cell embryos, followed by heat treatment, caused loss of head formation that was similar to that seen with CS2\/\u03b2\u2010catenin injection. Although a hormone\u2010inducible gene induction system already exists in <jats:italic>Xenopus<\/jats:italic>, our modified technique provides an alternative method for controlling temporal and spatial gene expression. Developmental Dynamics 232:369\u2013376, 2005. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20233","type":"journal-article","created":{"date-parts":[[2004,12,21]],"date-time":"2004-12-21T19:07:14Z","timestamp":1103656034000},"page":"369-376","source":"Crossref","is-referenced-by-count":14,"title":["Temporal and spatial manipulation of gene expression in <i>Xenopus<\/i> embryos by injection of heat shock promoter\u2010containing plasmids"],"prefix":"10.1002","volume":"232","author":[{"given":"Tatsuo","family":"Michiue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Asashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb02159.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.6.1279"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.2.471"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/39092"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1242\/dev.127.9.1953","article-title":"Laser\u2010induce gene expression in specific cells of transgenic zebrafish","volume":"127","author":"Halloran MC","year":"2000","journal-title":"Development"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","unstructured":"HeasmanJ KofronM WylieC.2000.\u03b2\u2010catenin signaling activity dissected in the earlyXenopusembryo: a novel antisense approach.Dev Biol.222:124\u2013134.","DOI":"10.1006\/dbio.2000.9720"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90536-2"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.21.4189"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02424465"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1279"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.10.3173"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1242\/dev.103.1.59","article-title":"Analysis of hsp 30, hsp 70 and ubiquitin gene expression in Xenopus laevis tadpoles","volume":"103","author":"Krone PH","year":"1988","journal-title":"Development"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1242\/dev.106.2.271","article-title":"Expression of microinjected hsp 70\/CAT and hsp 30\/CAT chimeric genes in developing Xenopus laevis embryos","volume":"106","author":"Krone PH","year":"1989","journal-title":"Development"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20037"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90249-5"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6289436"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00071a013"},{"key":"e_1_2_5_19_1","first-page":"39","volume-title":"Early development of Xenopus laevis: a laboratory manual","author":"Sive HL","year":"2000"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90070-F"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90069-B"},{"key":"e_1_2_5_22_1","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1242\/dev.124.11.2225","article-title":"Analysis of competence and of Brachyury autoinduction by use of hormone\u2010inducible Xbra","volume":"124","author":"Tada MA","year":"1997","journal-title":"Development"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00596-0"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.20233","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.20233","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:07:25Z","timestamp":1760346445000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.20233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,21]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["10.1002\/dvdy.20233"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.20233","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12,21]]}}}