{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:07:48Z","timestamp":1791306468022,"version":"4.3.1"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,14]],"date-time":"2005-03-14T00:00:00Z","timestamp":1110758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The neural and glial cells of the intrinsic ganglia of the enteric nervous system (ENS) are derived from the hindbrain neural crest at the vagal level. The <jats:italic>Hoxb3<\/jats:italic> gene is expressed in the vagal neural crest and in the enteric ganglia of the developing gut during embryogenesis. We have identified a <jats:italic>cis<\/jats:italic>\u2010acting enhancer element b3IIIa in the <jats:italic>Hoxb3<\/jats:italic> gene locus. In this study, by transgenic mice analysis, we examined the tissue specificity of the b3IIIa enhancer element using the <jats:italic>lacZ<\/jats:italic> reporter gene, with emphasis on the vagal neural crest cells and their derivatives in the developing gut. We found that the <jats:italic>b3IIIa\u2010lacZ<\/jats:italic> transgene marks only the vagal region and not the trunk or sacral region. Using cellular markers, we showed that the <jats:italic>b3IIIa\u2010lacZ<\/jats:italic> transgene was expressed in a subset of enteric neuroblasts during early development of the gut, and the expression was maintained in differentiated neurons of the myenteric plexus at later stages. The specificity of the b3IIIa enhancer in directing gene expression in the developing ENS was further supported by genetic analysis using the <jats:italic>Dom<\/jats:italic> mutant, a spontaneous mouse model of Hirschsprung's disease characterized by the absence of enteric ganglia in the distal gut. The colonization of <jats:italic>lacZ<\/jats:italic>\u2010expressing cells in the large intestine was incomplete in all the <jats:italic>Dom\/b3IIIa\u2010lacZ<\/jats:italic> hybrid mutants we examined. To our knowledge, this is the only vagal neural crest\u2010specific genetic regulatory element identified to date. This element could be used for a variety of genetic manipulations and in establishing transgenic mouse models for studying the development of the ENS. Developmental Dynamics 233:473\u2013483, 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20347","type":"journal-article","created":{"date-parts":[[2005,3,14]],"date-time":"2005-03-14T22:32:13Z","timestamp":1110839533000},"page":"473-483","source":"Crossref","is-referenced-by-count":24,"title":["<i>Hoxb3<\/i> vagal neural crest\u2010specific enhancer element for controlling enteric nervous system development"],"prefix":"10.1002","volume":"233","author":[{"given":"Kwok Keung","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuk Shan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai 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