{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:19:15Z","timestamp":1760782755018,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,5,5]],"date-time":"2005-05-05T00:00:00Z","timestamp":1115251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>Caenorhabditis elegans pos\u20101<\/jats:italic> gene encodes a zinc\u2010finger protein that is required for germline specification during embryogenesis. The maternally provided mRNA is translationally regulated both spatially and temporally during early development. We have cloned orthologs of <jats:italic>pos\u20101<\/jats:italic> from <jats:italic>C. briggsae<\/jats:italic> and <jats:italic>C. remanei<\/jats:italic>, two <jats:italic>Caenorhabditis<\/jats:italic> species that have diverged from <jats:italic>C. elegans<\/jats:italic> by approximately 20\u201340 million years. Two regions in the 3\u2032 untranslated region are highly conserved among all three species. We find that the <jats:italic>pos\u20101<\/jats:italic> RNA is expressed in the hermaphrodite and female gonads of <jats:italic>C. briggsae<\/jats:italic> and <jats:italic>C. remanei<\/jats:italic> but POS\u20101 protein is not detected at high levels in <jats:italic>C. briggsae<\/jats:italic> until the 2\u2010cell stage of embyrogenesis. The protein expression is restricted to the germline precursors of the embryo. We conclude that <jats:italic>pos\u20101<\/jats:italic> appears to be translationally regulated in <jats:italic>C. briggsae<\/jats:italic> as it is in <jats:italic>C. elegans<\/jats:italic> and speculate the conserved 3\u2032 UTR sequences may be involved. Developmental Dynamics 233:1006\u20131012, 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20421","type":"journal-article","created":{"date-parts":[[2005,5,6]],"date-time":"2005-05-06T14:28:04Z","timestamp":1115389684000},"page":"1006-1012","source":"Crossref","is-referenced-by-count":7,"title":["Cloning and expression analysis of <i>pos\u20101<\/i> in the nematodes <i>Caenorhabditis briggsae<\/i> and <i>Caenorhabditis remanei<\/i>"],"prefix":"10.1002","volume":"233","author":[{"given":"Jamie","family":"Konwerski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Senchuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Petty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Lahaie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer A.","family":"Schisa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/77.1.71"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2639304"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90311-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1093\/genetics\/161.1.99","article-title":"Levels of DNA polymorphism vary with mating system in the nematode genus Caenorhabditis","volume":"161","author":"Graustein A","year":"2002","journal-title":"Genetics"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/155.1.105"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81340-9"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.35.102401.090756"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1094"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403094101"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1125"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12451"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg087"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00469"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/157.2.639"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-142X.2002.02027.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.8.1287"},{"volume-title":"PAUP*: Phlyogenetic analysis using parsimony (*and other methods)","year":"2000","author":"Swofford DL","key":"e_1_2_5_18_1"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.1.1"},{"key":"e_1_2_5_20_1","unstructured":"WormBase Web site. Available at:http:\/\/wormbase.org release WS137. Accessed 1\/20\/2005."}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.20421","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.20421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T08:56:35Z","timestamp":1760345795000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.20421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5,5]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1002\/dvdy.20421"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.20421","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"type":"print","value":"1058-8388"},{"type":"electronic","value":"1097-0177"}],"subject":[],"published":{"date-parts":[[2005,5,5]]}}}