{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T05:34:44Z","timestamp":1772688884531,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,10,28]],"date-time":"2005-10-28T00:00:00Z","timestamp":1130457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Blowflies are the primary facultative agent in causing myiasis of domestic sheep in the whole world and, at the same time, it is an important tool for forensic medicine. Surprisingly, and in contrast to its importance, almost no data regarding the embryology and molecular markers are known for this insect. In this report, we present a detailed description of the blowfly <jats:italic>Lucilia sericata<\/jats:italic> embryogenesis and of imaginal disc development. The embryogenesis of <jats:italic>Lucilia<\/jats:italic> strongly resembles that of <jats:italic>Drosophila<\/jats:italic>, despite their apparent size difference. Moreover, imaginal disc development appears to be equally well conserved. Through cloning, expression, and functional studies, we show that the <jats:italic>Lucilia<\/jats:italic> Wingless (Wg) protein is highly conserved between the two species. We further show that parasegments are established in <jats:italic>Lucilia<\/jats:italic>, however, <jats:italic>engrailed<\/jats:italic> expression shows a more dynamic expression pattern than expected in comparison to <jats:italic>Drosophila<\/jats:italic>. Over\u2010expression of <jats:italic>Lucilia<\/jats:italic> Wingless in <jats:italic>Drosophila<\/jats:italic> shows <jats:italic>wingless<\/jats:italic>\u2010like wing phenotypes, suggesting that <jats:italic>Lucilia<\/jats:italic> Wingless blocks the signalling activity of <jats:italic>Drosophila<\/jats:italic> Wingless. Upon injection of <jats:italic>wg<\/jats:italic> dsRNA, we observe a \u201clawn of denticle\u201d phenotype, closely resembling that of <jats:italic>Drosophila<\/jats:italic>. Due to the large size of the insect, the distance over which Wingless exerts signalling activity is up to three times larger than in <jats:italic>Drosophila<\/jats:italic>, yet the consequences are very similar. Our data demonstrate long\u2010range <jats:italic>wingless<\/jats:italic> signaling mechanisms adapted for patterning large domains of naked cuticle and suggest signaling properties of <jats:italic>Lucilia<\/jats:italic> Wingless that are distinct from those of <jats:italic>Drosophila<\/jats:italic> Wingless. Developmental Dynamics 235:347\u2013360, 2006. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20632","type":"journal-article","created":{"date-parts":[[2005,10,28]],"date-time":"2005-10-28T17:16:31Z","timestamp":1130519791000},"page":"347-360","source":"Crossref","is-referenced-by-count":17,"title":["Wingless signaling in a large insect, the blowfly <i>Lucilia sericata<\/i>: A beautiful example of evolutionary developmental biology"],"prefix":"10.1002","volume":"235","author":[{"given":"Katja","family":"Mellenthin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalid","family":"Fahmy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reda 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