{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:50:59Z","timestamp":1789678259938,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,5,5]],"date-time":"2006-05-05T00:00:00Z","timestamp":1146787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The novel gene <jats:italic>ashwin<\/jats:italic> was isolated in a differential display screen for genes activated or up\u2010regulated early in neural specification. <jats:italic>ashwin<\/jats:italic> is expressed maternally and zygotically, and it is up\u2010regulated in the neural ectoderm after the midgastrula stage. It is expressed in the neural plate and later in the embryonic brain, eyes, and spinal cord. Overexpression of <jats:italic>ashwin<\/jats:italic> in whole embryos leads to anterior truncations and other defects. However, a second Organizer does not form, and the secondary axial structures may result from splitting of the Organizer, rather than axis duplication. Morpholino oligonucleotide\u2010mediated reduction in <jats:italic>ashwin<\/jats:italic> expression leads to lethality or abnormalities in gastrulation, as well as significant apoptosis in midgastrula embryos. Apoptosis is also observed in midgastrula embryos overexpressing <jats:italic>ashwin<\/jats:italic>. Coexpression of <jats:italic>ashwin<\/jats:italic> with the bone morphogenetic protein\u20104 antagonist <jats:italic>noggin<\/jats:italic> has a synergistic effect on neural\u2010specific gene expression in isolated animal cap ectoderm. Ashwin has no previously characterized domains, although two nuclear localization signals can be identified. Orthologues have been identified in the human, mouse, chicken, and pufferfish genomes. Our results suggest that <jats:italic>ashwin<\/jats:italic> regulates cell survival and anteroposterior patterning. Developmental Dynamics 235:1895\u20131907, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20834","type":"journal-article","created":{"date-parts":[[2006,5,5]],"date-time":"2006-05-05T14:22:55Z","timestamp":1146838975000},"page":"1895-1907","source":"Crossref","is-referenced-by-count":12,"title":["Novel gene <i>ashwin<\/i> functions in <i>Xenopus<\/i> cell survival and anteroposterior patterning"],"prefix":"10.1002","volume":"235","author":[{"given":"Sonali S.","family":"Patil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara B.","family":"Alexander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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