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We show that this method detects predominantly mature miRNAs rather than the miRNA precursors or primary transcripts. We have determined expression patterns for several miRNAs expressed in the developing and adult nervous system, including miR\u2010124a, miR\u20109, miR\u201092, and miR\u2010204. Whereas miR\u2010124a is expressed in neurons, miR\u20109 is expressed in neural progenitors and some neurons, and miR\u2010204 is expressed in the choroid plexus, retinal pigment epithelium, and ciliary body. miR\u2010204 is located in an intron of the TRPM3 gene, and the TRPM3 mRNA is coexpressed with miR\u2010204 in the choroid plexus. We also find that primary transcripts for miR\u2010124a and miR\u20109 genes are expressed in patterns similar to their respective mature miRNAs. The ability to visualize expression of specific miRNAs in embryos and tissues should aid studies on miRNA function. 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