{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:02:04Z","timestamp":1762160524780,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,6,21]],"date-time":"2006-06-21T00:00:00Z","timestamp":1150848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To explore <jats:italic>Bapx1<\/jats:italic> homeobox gene function in embryonic control of development, we employed a gain\u2010of\u2010function approach to complement our previous loss\u2010of\u2010function mutant analysis. We show that transgenic mice overexpressing <jats:italic>Bapx1<\/jats:italic> are affected by skeletal defects including hindlimb preaxial polydactyly and tibial hypoplasia. <jats:italic>Bapx1<\/jats:italic> overexpression generates limb anteroposterior patterning defects including induction of <jats:italic>Shh<\/jats:italic> signaling and ectopic activation of functions downstream of <jats:italic>Shh<\/jats:italic> signaling into the anterior region of the autopod. Moreover, <jats:italic>Bapx1<\/jats:italic> overexpression stimulates formation of limb prechondrogenic condensations. We also show that <jats:italic>Shh<\/jats:italic> is reciprocally able to activate <jats:italic>Bapx1<\/jats:italic> expression in mouse embryos as the orthologous <jats:italic>hedgehog<\/jats:italic> (<jats:italic>hh<\/jats:italic>) does with the <jats:italic>bagpipe\/Bapx1<\/jats:italic> gene in <jats:italic>Drosophila<\/jats:italic>. Our results indicate that <jats:italic>Bapx1<\/jats:italic> can modulate appendicular skeletal formation, that the genetic hierarchy between <jats:italic>Shh\/hh<\/jats:italic> and <jats:italic>Bapx1\/bagpipe<\/jats:italic> has been conserved during evolution, and that in mouse embryos these two genes can influence one another in a genetically reciprocal manner. We conclude that it is reasonable to expect overexpression of <jats:italic>Bapx1<\/jats:italic> in certain forms of polydactyly. Developmental Dynamics 235:2483\u20132492, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.20867","type":"journal-article","created":{"date-parts":[[2006,6,21]],"date-time":"2006-06-21T13:03:54Z","timestamp":1150895034000},"page":"2483-2492","source":"Crossref","is-referenced-by-count":8,"title":["<i>Bapx1<\/i> homeobox gene gain\u2010of\u2010function mice show preaxial polydactyly and activated <i>Shh<\/i> signaling in the developing limb"],"prefix":"10.1002","volume":"235","author":[{"given":"Carla","family":"Tribioli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Lufkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.2000.00339.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1017802"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.24.3183"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.0010"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(97)00656-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a109486"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1242\/dev.127.11.2461","article-title":"The 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