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Lymphangiogenesis requires lymphatic endothelial cell (LEC) differentiation, proliferation, migration, and co\u2010ordinated network formation, yet the transcriptional regulators underpinning these processes remain to be fully understood. The transcription factor MAFB was recently identified as essential for lymphangiogenesis in zebrafish and in cultured human LECs. MAFB is activated in response to VEGFC\u2010VEGFR3 signaling and acts as a downstream effector. However, it remains unclear if the role of MAFB in lymphatic development is conserved in the mammalian embryo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We generated a <jats:italic>Mafb<\/jats:italic> loss\u2010of\u2010function mouse using CRISPR\/Cas9 gene editing. <jats:italic>Mafb<\/jats:italic> mutant mice presented with perinatal lethality associated with cyanosis. We identify a role for MAFB in modifying lymphatic network morphogenesis in the developing dermis, as well as developing and postnatal diaphragm. Furthermore, mutant vessels displayed excessive smooth muscle cell coverage, suggestive of a defect in the maturation of lymphatic networks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This work confirms a conserved role for MAFB in murine lymphatics that is subtle and modulatory and may suggest redundancy in MAF family transcription factors during lymphangiogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.209","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T05:45:09Z","timestamp":1591854309000},"page":"1201-1216","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>MAFB<\/scp> modulates the maturation of lymphatic vascular networks in mice"],"prefix":"10.1002","volume":"249","author":[{"given":"Maria","family":"Rondon\u2010Galeano","sequence":"first","affiliation":[{"name":"Division of Genomics of Development and Disease Institute for Molecular Bioscience, The University of Queensland  St Lucia, Brisbane Queensland Australia"},{"name":"Organogenesis and Cancer Program, Peter MacCallum Cancer Centre  Melbourne Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renae","family":"Skoczylas","sequence":"additional","affiliation":[{"name":"Division of Genomics of Development and Disease Institute for Molecular Bioscience, The University of Queensland  St Lucia, Brisbane Queensland Australia"},{"name":"Department of Immunology, Genetics and Pathology Uppsala University  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil I.","family":"Bower","sequence":"additional","affiliation":[{"name":"Division of Genomics of Development and Disease Institute for Molecular Bioscience, The University of Queensland  St Lucia, Brisbane Queensland 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