{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:36:25Z","timestamp":1760402185663,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T00:00:00Z","timestamp":1161907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cux\u20101 is a murine homeobox gene structurally related to <jats:italic>Drosophila<\/jats:italic> cut. Cux\u20101 is highly expressed in the nephrogenic zone of the developing kidney, where its expression coincides with cell proliferation. Cux\u20101 functions as a transcriptional repressor of the cyclin kinase inhibitors (CKI) p21 and p27. Cux\u20101 DNA binding activity is negatively regulated by phosphorylation, and dephosphorylation of Cux\u20101 results in increased DNA binding. Transgenic mice ectopically expressing Cux\u20101 develop renal hyperplasia associated with the down\u2010regulation of the CKI p27. Calcineurin A (CnA) \u03b1 (\u2212\/\u2212) mice display renal hypoplasia associated with the ectopic expression of p27. CnA is a serine\/threonine phosphatase activated by intracellular calcium. Inhibiting CnA with cyclosporin A (CsA) leads to nephron deficit in rat metanephric organ cultures and apoptosis in various renal cell lines. To determine whether the ectopic expression of p27 in CnA\u2010\u03b1 \u2212\/\u2212 kidneys results from the down\u2010regulation of Cux\u20101, metanephroi from embryonic Cux\u20101 transgenic and wild\u2010type mice were harvested and cultured with CsA for 5 days. CsA treatment significantly inhibited growth of wild\u2010type metanephroi. In contrast, CsA\u2010treated Cux\u20101 transgenic kidney cultures were not growth inhibited, but showed high levels of cell proliferation in the nephrogenic zone. Moreover, in CsA\u2010treated Cux\u20101 transgenic kidney cultures, p27 was not expressed in the nephrogenic zone, but only up\u2010regulated in maturing glomeruli and tubules. Taken together, our results demonstrate that ectopic expression of Cux\u20101 can rescue the effects of CsA inhibition of CnA and suggest that Cux\u20101 may be regulated by calcineurin A. Developmental Dynamics 236:184\u2013191, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21003","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T16:46:51Z","timestamp":1161967611000},"page":"184-191","source":"Crossref","is-referenced-by-count":6,"title":["Ectopic expression of the homeobox gene Cux\u20101 rescues calcineurin inhibition in mouse embryonic kidney cultures"],"prefix":"10.1002","volume":"236","author":[{"given":"Neal I.","family":"Alcalay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer G.","family":"Brantley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhulika","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Gooch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory B.","family":"Vanden Heuvel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.40.24862"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.16.4680"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.5.2561"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.6.4251"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63430-X"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(96)00221-7"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0636"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.10.5346"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.2.529"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00485-1"},{"key":"e_1_2_6_12_1","first-page":"90","article-title":"Differentiation of epithelial foot processes and filtration slits: sequential appearance of occluding junctions, epithelial polyanion, and slit membranes in developing glomeruli","volume":"39","author":"Reeves W","year":"1978","journal-title":"Lab Invest"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.4.1483"},{"key":"e_1_2_6_14_1","first-page":"497A","article-title":"Increased proliferation and apoptosis of mesangial cells isolated from Cux\u20101 transgenic mice","volume":"15","author":"Sharma M","year":"2004","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000095637.13193.89"},{"key":"e_1_2_6_16_1","first-page":"168","volume-title":"The house mouse: development and normal stages from fertilization to 4 weeks of age","author":"Theiler K","year":"1972"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.3.881"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.336"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1993.tb17137.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02723053"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.21003","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.21003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:25:57Z","timestamp":1760361957000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.21003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10,27]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/dvdy.21003"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.21003","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"type":"print","value":"1058-8388"},{"type":"electronic","value":"1097-0177"}],"subject":[],"published":{"date-parts":[[2006,10,27]]}}}