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Because <jats:italic>Hoxa13<\/jats:italic> homozygous mutant mice also exhibit hypospadias and GT vessel expansion, we examined whether genes playing a role in angiogenesis exhibit reduced expression in the GT. From this analysis, reductions in <jats:italic>EphA6<\/jats:italic> and <jats:italic>EphA7<\/jats:italic> were detected. Characterization of <jats:italic>EphA6<\/jats:italic> and <jats:italic>EphA7<\/jats:italic> expression in the GT confirmed colocalization with HOXA13 in the GT vascular endothelia. Analysis of the <jats:italic>EphA6<\/jats:italic> and <jats:italic>EphA7<\/jats:italic> promoter regions revealed a series of highly conserved <jats:italic>cis<\/jats:italic>\u2010regulatory elements bound by HOXA13 with high affinity. GT chromatin immunoprecipitation confirmed that HOXA13 binds these gene\u2010regulatory elements in vivo. In vitro, HOXA13 activates gene expression through the <jats:italic>EphA6<\/jats:italic> and <jats:italic>EphA7<\/jats:italic> gene\u2010regulatory elements. Together these findings indicate that HOXA13 directly regulates <jats:italic>EphA6<\/jats:italic> and <jats:italic>EphA7<\/jats:italic> in the developing GT and identifies the GT vascular endothelia as a novel site for HOXA13\u2010dependent expression of <jats:italic>EphA6<\/jats:italic> and <jats:italic>EphA7<\/jats:italic>. Developmental Dynamics 236:951\u2013960, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21077","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T11:40:34Z","timestamp":1171539634000},"page":"951-960","source":"Crossref","is-referenced-by-count":31,"title":["HOXA13 directly regulates <i>EphA6<\/i> and <i>EphA7<\/i> expression in the genital tubercle vascular endothelia"],"prefix":"10.1002","volume":"236","author":[{"given":"Carley A.","family":"Shaut","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chie","family":"Saneyoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily A.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy M.","family":"Knosp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane R.","family":"Sexton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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