{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:12:09Z","timestamp":1760404329046,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,6,18]],"date-time":"2007-06-18T00:00:00Z","timestamp":1182124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The anterior segment of the eye includes such structures as the cornea, lens, iris, and ciliary body and is essential for many visual and physiological functions of the eye. The zebrafish <jats:italic>gelsolin\u2010like 1<\/jats:italic> (<jats:italic>gsnl1<\/jats:italic>) gene encodes an actin regulatory protein and is expressed in the anterior segment of the eye. We report the transgenic analyses of the <jats:italic>gsnl1<\/jats:italic> promoter and enhancer that are required for expression in the anterior segment of the eye. A 6.4\u2010kb genomic fragment upstream from the translation initiation site (ATG) was capable of driving green fluorescent protein (GFP) expression in transient transgenic embryos and stable transgenic adult fish, which mimics the endogenous <jats:italic>gsnl1<\/jats:italic> expression. The GFP expression was localized in the corneal epithelium (CE) and the annular ligament (AL) at the iridocorneal angle. A unique enhancer for each of these two tissues was identified at 3.7\u2010kb upstream from the ATG. The 60\u2010bp AL and 25\u2010bp CE enhancers were separated by 100\u2010bp and functioned independently from each other. Deletion analysis indicated that the proximal promoter was located 1.6\u2010kb upstream from the ATG. Stable GFP transgenic lines were established for future studies of genetic regulation in the anterior segment of the fish eye. Developmental Dynamics 236:1929\u20131938, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21197","type":"journal-article","created":{"date-parts":[[2007,6,18]],"date-time":"2007-06-18T18:17:19Z","timestamp":1182190639000},"page":"1929-1938","source":"Crossref","is-referenced-by-count":13,"title":["Transgenic analysis of the anterior eye\u2010specific enhancers of the zebrafish <i>gelsolin<\/i>\u2010<i>like 1<\/i> (<i>gsnl1<\/i>) gene"],"prefix":"10.1002","volume":"236","author":[{"given":"Shunichi","family":"Yoshikawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan","family":"Norcom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Yee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinping C.","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2174572"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00026.2002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0691"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1042\/bj2720827","article-title":"Gelsolin variant (Asn\u2010187) in familial amyloidosis, Finnish type","volume":"15","author":"Ghiso J","year":"1990","journal-title":"Biochem J"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.041865dg"},{"key":"e_1_2_6_8_1","first-page":"522","article-title":"Finnish type of familial amyloidosis: cosegregation of Asp187Asn mutation of gelsolin with disease in three large families","volume":"49","author":"Hiltunen T","year":"1991","journal-title":"Am J Hum Genet"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2006.02.006"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0634473100"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2004.04.002"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.06.005"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90935-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.6.1865"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80072-Q"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00932-3"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4225-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-5-12"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(04)77027-2"},{"key":"e_1_2_6_20_1","first-page":"1083","article-title":"Expressed sequence tag analysis of zebrafish eye tissues for NEIBank","volume":"11","author":"Vihtelic TS","year":"2005","journal-title":"Mol Vis"},{"volume-title":"The zebrafish book. 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