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Herein, we study the changes undergone by the thymus of EphB\u2010deficient mice throughout their life.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immune alterations occurring throughout life were more severe in mutant than in wild\u2010type (WT) mice. Mutant thymuses exhibit lower cellularity than WT ones, as well as lower proportions of early thymic progenitors cells and double\u2010positive (CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>) thymocytes, but higher of double\u2010negative (CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>) and single\u2010positive (CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>, CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>+<\/jats:sup>) cells. Throughout life, CD4<jats:sup>+<\/jats:sup> na\u00efve cells decreased particularly in mutant mice. In correlation, memory T cells, largely CD8<jats:sup>+<\/jats:sup> cells, increased. Aged thymic epithelium undergoes changes including appearance of big epithelial free areas, decrease of K8<jats:sup>+<\/jats:sup>K5<jats:sup>\u2212<\/jats:sup> areas, which, however, contain higher proportions of Ly51<jats:sup>+<\/jats:sup>UEA1<jats:sup>\u2212<\/jats:sup> cortical epithelial cells, in correlation with reduced Aire<jats:sup>+<\/jats:sup> medullary epithelial cells. Also, aged thymuses particularly those derived from mutant mice exhibited increased collagen IV, fat\u2010storing cells, and connective cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The absence of EphB accelerates the alterations undergone throughout life by both thymic epithelium and thymocytes, and the proportions of peripheral na\u00efve and memory T cells, all of which are hallmarks of immune aging.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.212","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T09:30:52Z","timestamp":1591522252000},"page":"1243-1258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Thymus aging in mice deficient in either <scp>EphB2<\/scp> or <scp>EphB3<\/scp>, two master regulators of thymic epithelium 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