{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T11:14:10Z","timestamp":1767611650046,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41976092","31672628"],"award-info":[{"award-number":["41976092","31672628"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The nuclear progesterone receptor (Pgr) is a ligand\u2010dependent transcription factor primarily responsible for mediating progesterone actions relevant for reproduction across vertebrates. Information on the cellular localization of Pgr expression in the reproductive system is required for developing a comprehensive approach to elucidate the role of Pgr in reproduction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We generated transgenic zebrafish <jats:italic>Tg(pgr:eGFP)<\/jats:italic> that express enhanced green fluorescent protein (eGFP) driven by promoter sequence of <jats:italic>pgr<\/jats:italic> gene. The tissue distribution pattern of <jats:italic>egfp<\/jats:italic> mRNA is consistent with the <jats:italic>pgr<\/jats:italic> mRNA expression in <jats:italic>Tg(pgr:eGFP)<\/jats:italic>. In the pituitary, GFP signals are found in the proximal pars distalis. In order to better discern the cellular localization of GFP signals in gonads, <jats:italic>Tg(pgr:eGFP)<\/jats:italic> line was crossed with <jats:italic>Tg(gsdf:nfsB\u2010mCherry)<\/jats:italic> line, specifically expressing nitroreductase\u2010mCherry fusion protein in granulosa and Sertoli cells in ovary and testis, respectively. Imaging of testis tissue showed that GFP expression was confined to Leydig cells. In the ovary, GFP expression colocalized with the mCherry signal in granulosa cells. Intriguingly, we also identified some non\u2010granulosa cells close to where blood vessels branched, expressing stronger GFP signals than granulosa cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Analyzing <jats:italic>Tg(pgr:eGFP)<\/jats:italic> expression in zebrafish provided leads toward new routes to study the role of Pgr in reproduction.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.213","type":"journal-article","created":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T09:34:12Z","timestamp":1591522452000},"page":"1365-1376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>GFP<\/scp> expression pattern in pituitary and gonads under the control of nuclear progesterone receptor promoter in transgenic zebrafish"],"prefix":"10.1002","volume":"249","author":[{"given":"Jing","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences Xiamen University  Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences Xiamen University  Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences Xiamen University  Xiamen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan Shu","family":"Hong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences Xiamen University  Xiamen 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